Transcriptomics,Multiomics

Dataset Information

35

Transcription profiling by array of yeast treated with clioquinol


ABSTRACT: Clioquinol (CQ or iodochlorhydroxyquin, 5-chloro-7-iodo-8-hydroxyquinoline) is a hydrophobic chelator of copper, zinc, and iron. It was extensively used as an antibiotic for the treatment of diarrhea and skin infection in the mid-1900s, but then withdrawn because it was reported to be associated with subacute myelo-optic neuropathy (SMON) in Japan. Interest in this drug was recently revived as it was shown that CQ specifically kills cancer cells and significantly decreases Abeta level. Nevertheless, the underlying mechanisms of CQ drug effects and side effects are still unclear. We used yeast, Saccharomyces cerevisiae, as a model to study how CQ affects molecular and cellular functions. Genechip analysis was utilized to examine the effect of CQ on gene expression at the genomic level. To study gene expression profiles of yeast treated with CQ, yeast cells were treated with CQ or DMSO for 5 hours and RNAs were extracted for microarray analysis.

REANALYSED by: E-GEOD-17257

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Bing Zhou  Chenghua Li     

PROVIDER: E-GEOD-17257 | ArrayExpress | 2015-09-04

SECONDARY ACCESSION(S): GSE17257PRJNA118941

REPOSITORIES: GEO, ArrayExpress

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Publications

The metal chelating and chaperoning effects of clioquinol: insights from yeast studies.

Li Chenghua C   Wang Juan J   Zhou Bing B  

Journal of Alzheimer's disease : JAD 20100101 4


Clioquinol (CQ), a once popular antibiotic, was used to inhibit the growth of microorganisms. Recently, CQ and its analog PBT2 have shown encouraging effects in the animal and clinical trials for Alzheimer's disease (AD). However, the mechanism by which this class of molecules works remains controversial. In this work, we used the yeast Saccharomyces cerevisiae as a model to study how CQ affects molecular and cellular functions and particularly, copper, iron, and zinc homeostasis. We observed a  ...[more]

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