Dataset Information


Kc167 Replication Origins

ABSTRACT: modENCODE_submission_709 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see Project Goal: Early origins of replication were identified by treating cells with hydroxyurea (HU), a potent inhibitor of nucleotide synthesis, in the presence of the nucleotide analogue BrdU. Treatment of synchronized Kc167 cells with HU stalls replication forks and activates the intra S-phase checkpoint, thereby limiting BrdU incorporation to those sequences immediately adjacent to early activating replication origins. BrdU enriched sequences surrounding early origins of replication are then enriched by immunoprecipitation with an anti-BrdU antibody. Early origins are then detected by hybridization to Agilent genomic tiling arrays. Peaks are called using MA2C ( Keywords: CHIP-chip For data usage terms and conditions, please refer to and EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: Kc167; Tissue: embryo-derived cell-line; Genotype: se/e; Sex: Female NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line Kc167

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Heather MacAlpine   David MacAlpine  Leyna DeNapoli  DCC modENCODE 

PROVIDER: E-GEOD-17285 | ArrayExpress | 2010-05-15



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Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading.

MacAlpine Heather K HK   Gordân Raluca R   Powell Sara K SK   Hartemink Alexander J AJ   MacAlpine David M DM  

Genome Research 20091207 2

The origin recognition complex (ORC) is an essential DNA replication initiation factor conserved in all eukaryotes. In Saccharomyces cerevisiae, ORC binds to specific DNA elements; however, in higher eukaryotes, ORC exhibits little sequence specificity in vitro or in vivo. We investigated the genome-wide distribution of ORC in Drosophila and found that ORC localizes to specific chromosomal locations in the absence of any discernible simple motif. Although no clear sequence motif emerged, we were  ...[more]

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