Transcriptomics

Dataset Information

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MiRNA expression in LNCaP, PC3, Du-145 and RWPE-1 cell lines


ABSTRACT: MiRNAs are small non-coding RNAs that regulate the expression of specific mRNA targets mainly by translational repression, mRNA deadenylation or cleavage. This series is meant to identify miRNAs deregulated in prostate cancer (PCa) by comparing the PCa cell lines LNCaP, PC3 and Du-145 to the normal prostate epithelial cell line RWPE-1. We analyzed three arrays each for LNCaP, PC3, Du-145 and RWPE-1 cell lines

ORGANISM(S): Homo sapiens  

SUBMITTER: Martin v Bergen  Gerald Verhaegh   Nora Mörbt   Friedemann Horn   Janina Tomm   Kristin Reiche   Kerstin Boll   Katharina Schutt   Jack Schalken   Jörg Hackermüller   Antje K Kretzschmar    

PROVIDER: E-GEOD-17317 | ArrayExpress | 2012-03-08

SECONDARY ACCESSION(S): GSE17317PRJNA123605

REPOSITORIES: GEO, ArrayExpress

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Publications

MiR-130a, miR-203 and miR-205 jointly repress key oncogenic pathways and are downregulated in prostate carcinoma.

Boll K K   Reiche K K   Kasack K K   Mörbt N N   Kretzschmar A K AK   Tomm J M JM   Verhaegh G G   Schalken J J   von Bergen M M   Horn F F   Hackermüller J J  

Oncogene 20120305 3


With ∼30 000 deaths annually in the United States, prostate cancer (PCa) is a major oncologic disease. Here we show that the microRNAs miR-130a, miR-203 and miR-205 jointly interfere with the two major oncogenic pathways in prostate carcinoma and are downregulated in cancer tissue. Using transcriptomics we show that the microRNAs repress several gene products known to be overexpressed in this cancer. Argonaute 2 (AGO2) co-immunoprecipitation, reporter assays and western blot analysis demonstrate  ...[more]

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