Transcriptomics

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Gene expression profile of human prostate cancer cells transfected with Stat3 siRNA or Stat5a/b siRNA


ABSTRACT: Identification of the molecular changes that promote viability and metastatic behaviour of prostate cancer cells is critical for the development of improved therapeutic interventions for prostate cancer. Stat5a/b and Stat3 are both constitutively active in locally-confined and advanced prostate cancer, and both transcription factors have been reported to be critical for the viability and growth of prostate cancer cells. We used microarrays to compare gene expression profiles regulated by Stat5a/b vs. Stat3 in human prostate cancer cells. DU145 and CWR22Rv1 human prostate cancer cells were transfected with Stat3 siRNA, Stat5a/b siRNA or scramble siRNA as control. After 48 h, the cells were harvested and total RNA was prepared for Affymetrix microarrays.

REANALYSED by: E-GEOD-17482

ORGANISM(S): Homo sapiens  

SUBMITTER: Paolo Fortina   Gloria Bonucelli  Lukas Bubendorf  Marja T Nevalainen  Lei Gu  Jacqueline Lutz  Benjamin Leiby  Marja Nevalainen  Ayush Dagvadorj  Michael P Lisanti  Abhijit Dasgupta  Sankar Addya  Terry M Hyslop  Johng Rhimm 

PROVIDER: E-GEOD-17482 | ArrayExpress | 2010-05-27

SECONDARY ACCESSION(S): GSE17482PRJNA118767

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcription factor Stat3 stimulates metastatic behavior of human prostate cancer cells in vivo, whereas Stat5b has a preferential role in the promotion of prostate cancer cell viability and tumor growth.

Gu Lei L   Dagvadorj Ayush A   Lutz Jacqueline J   Leiby Benjamin B   Bonuccelli Gloria G   Lisanti Michael P MP   Addya Sankar S   Fortina Paolo P   Dasgupta Abhijit A   Hyslop Terry T   Bubendorf Lukas L   Nevalainen Marja T MT  

The American journal of pathology 20100218 4


Identification of the molecular changes that promote viability and metastatic behavior of prostate cancer is critical for the development of improved therapeutic interventions. Stat5a/b and Stat3 are both constitutively active in locally-confined and advanced prostate cancer, and both transcription factors have been reported to be critical for the viability of prostate cancer cells. We recently showed that Stat3 promotes metastatic behavior of human prostate cancer cells not only in vitro but al  ...[more]

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