Transcriptomics

Dataset Information

7

Gene expression programs in PBMCs elicited by 6 major mediators of the immune response.


ABSTRACT: Abstract: Background Interferons are key modulators of the immune system, and are central to the control of many diseases. The response of immune cells to stimuli in complex populations is the product of direct and indirect effects, homotypic and heterotypic cell interactions. Dissecting the global transcriptional profiles of immune cell populations may provide insights into this regulatory interplay. The host transcriptional response may also be useful in discriminating between disease states, and in understanding pathophysiology. The transcriptional programs of cell populations in health therefore provide a paradigm for deconvoluting disease-associated gene expression profiles. Results: We used human cDNA microarrays to (1) compare the gene expression programs in human peripheral blood mononuclear cells (PBMCs) elicited by 6 major mediators of the immune response: interferons a, b, w and g, IL12 and TNFa; and (2) characterise the transcriptional responses of purified immune cell populations (CD4+ and CD8+ T cells, B cells, NK cells and monocytes) to IFNg stimulation. We defined a highly stereotyped response to type I interferons, while responses to IFNg and IL12 were largely restricted to a subset of type I interferon-inducible genes. TNFa stimulation resulted in a distinct pattern of gene expression. Cell type-specific transcriptional programs were identified, highlighting the pronounced response of monocytes to IFNg, and emergent properties associated with IFN-mediated activation of mixed cell populations. Conclusions: This information provides a detailed view of cellular activation by immune mediators, and contributes an interpretive framework for the detection and definition of host immune responses in a variety of disease settings. This SuperSeries is composed of the following subset Series: GSE16251: PBMC Cytokine Comparison GSE16252: PBMC IFNg Dose Reponse GSE16253: PBMC Cell Subset Stimulation Refer to individual Series

ORGANISM(S): Homo sapiens  

SUBMITTER: Simon Waddell  

PROVIDER: E-GEOD-17762 | ArrayExpress | 2010-03-21

SECONDARY ACCESSION(S): GSE17762PRJNA118307

REPOSITORIES: GEO, ArrayExpress

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Publications

Dissecting interferon-induced transcriptional programs in human peripheral blood cells.

Waddell Simon J SJ   Popper Stephen J SJ   Rubins Kathleen H KH   Griffiths Michael J MJ   Brown Patrick O PO   Levin Michael M   Relman David A DA  

PloS one 20100322 3


Interferons are key modulators of the immune system, and are central to the control of many diseases. The response of immune cells to stimuli in complex populations is the product of direct and indirect effects, and of homotypic and heterotypic cell interactions. Dissecting the global transcriptional profiles of immune cell populations may provide insights into this regulatory interplay. The host transcriptional response may also be useful in discriminating between disease states, and in underst  ...[more]

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