Transcriptomics

Dataset Information

4

ChIP-chip of Geobacter sulfurreducens PCA with antibody against RpoN under various conditions.


ABSTRACT: We applied a ChIP-chip approach to elucidate the σN regulon experimentally under different growth conditions. This technique localizes DNA fragments within σN complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A nine ChIP-chip study using immunoprecipitated DNA (IP-DNA) from three separate culture conditions. The high-density oligonucleotide tiling arrays used were consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G.sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures)

ORGANISM(S): Geobacter sulfurreducens PCA  

SUBMITTER: Derek Lovley   Bernhard Palsson  Jessica De Ingeniis  Bernhard O Palsson  Karsten Zengler  Vasiliy A Portnoy  Yu Qiu  Byung-Kwan Cho 

PROVIDER: E-GEOD-17837 | ArrayExpress | 2010-06-30

SECONDARY ACCESSION(S): GSE17837PRJNA123289

REPOSITORIES: GEO, ArrayExpress

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