Transcriptomics

Dataset Information

120

Investigation of barley brittle stem mutants


ABSTRACT: The barley brittle stem mutants, fs2, designated X054 and M245, have reduced levels of cellulose compared with their isogenic parents Ohichi and Shiroseto. A custom-designed microarray, based on Agilent technology and including genes involved in cell wall metabolism, was used to compare transcript levels in the mutant and parental lines. For both mutants, the microarray revealed a marked decrease in mRNA for the HvCesA4 cellulose synthase gene in specific zones of stem internodes, and this was confirmed by quantitative PCR. Genes expression was measured for the upper and lower zones from the 4th internodes of stems. Plant were at Zadocks developmental stage 49. Gene expression was compared between mutants and their wildtype parents.

ORGANISM(S): Hordeum vulgare  

SUBMITTER: Antony Bacic   Scott V Tingey  Carl R Simmons  Kanwarpal S Dhugga  Mary Beatty  Jillian Taylor  Ute Baumann  Neil J Shirley  Geoffrey B Fincher  Andrew J Harvey  Gang Ma  Filomena Pettolino  J A Rafalski  Rachel A Burton 

PROVIDER: E-GEOD-17873 | ArrayExpress | 2010-07-23

SECONDARY ACCESSION(S): GSE17873PRJNA119987

REPOSITORIES: GEO, ArrayExpress

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Publications

A customized gene expression microarray reveals that the brittle stem phenotype fs2 of barley is attributable to a retroelement in the HvCesA4 cellulose synthase gene.

Burton Rachel A RA   Ma Gang G   Baumann Ute U   Harvey Andrew J AJ   Shirley Neil J NJ   Taylor Jillian J   Pettolino Filomena F   Bacic Antony A   Beatty Mary M   Simmons Carl R CR   Dhugga Kanwarpal S KS   Rafalski J Antoni JA   Tingey Scott V SV   Fincher Geoffrey B GB  

Plant physiology 20100607 4


The barley (Hordeum vulgare) brittle stem mutants, fs2, designated X054 and M245, have reduced levels of crystalline cellulose compared with their parental lines Ohichi and Shiroseto. A custom-designed microarray, based on long oligonucleotide technology and including genes involved in cell wall metabolism, revealed that transcript levels of very few genes were altered in the elongation zone of stem internodes, but these included a marked decrease in mRNA for the HvCesA4 cellulose synthase gene  ...[more]

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