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Fibrinogen-reactive T cells transfer disease to naïve mice

ABSTRACT: Splenocytes harvested from mice with FIA were cultured in vitro and stimulated with 0.01 mg/ml fibrinogen for 3 days. Enriched T cells were transferred into 6-week-old naïve SJL mice, which developed visible signs of arthritis within 2 weeks. Synovial array profiling of plasma from the arthritic recipient mice demonstrated autoreactive B cell responses against peptides representing native fibrinogen, and further spreading of the responses to target collagen type V, cartilage gp39, and citrullinated vimentin. Custom-spotted protein slides were with plasma samples from individual mice. Four slides were probed with plasma derived from naïve mice and five slides were probed with plasma derived from mice injected with FIA T cells.

ORGANISM(S): Mus musculus  

SUBMITTER: Maya J BenBarak   Peggy P Ho  Lowen Y Lee  Wolfgang Hueber  Orr Sharpe  Beren H Tomooka  Lawrence Steinman  William H Robinson  Xiaoyan Zhao  Ricardo T Paniagua  William Robinson  Piyanka E Chandra 

PROVIDER: E-GEOD-18025 | ArrayExpress | 2010-05-05



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Autoimmunity against fibrinogen mediates inflammatory arthritis in mice.

Ho Peggy P PP   Lee Lowen Y LY   Zhao Xiaoyan X   Tomooka Beren H BH   Paniagua Ricardo T RT   Sharpe Orr O   BenBarak Maya J MJ   Chandra Piyanka E PE   Hueber Wolfgang W   Steinman Lawrence L   Robinson William H WH  

Journal of immunology (Baltimore, Md. : 1950) 20091130 1

Rheumatoid arthritis (RA) is an autoimmune synovitis characterized by the presence of anticitrullinated protein Abs, although the exact targets and role of anticitrullinated protein autoimmunity in the pathogenesis of RA remain to be defined. Fibrinogen, which can be citrullinated, has recently emerged as a candidate autoantigen. To determine whether autoimmunity against fibrinogen can mediate inflammatory arthritis, we immunized a variety of common mouse strains with fibrinogen and found that D  ...[more]

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