Transcriptomics

Dataset Information

420

High-throughput sequencing of small RNAs from different developmental stages of tomato fruits


ABSTRACT: Comparison of endogenous small RNA profiles from different developmental stages of tomato fruits Size fractionated small RNA from total RNA extracts was ligated to adaptors, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

ORGANISM(S): Solanum lycopersicum  

SUBMITTER: Frank Schwach   Tamas Dalmay  Irina Mohorianu  Vincent Moulton  Simon Moxon  Runchun Jing 

PROVIDER: E-GEOD-18110 | ArrayExpress | 2011-06-02

SECONDARY ACCESSION(S): SRP002313GSE18110PRJNA119405

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Profiling of short RNAs during fleshy fruit development reveals stage-specific sRNAome expression patterns.

Mohorianu Irina I   Schwach Frank F   Jing Runchun R   Lopez-Gomollon Sara S   Moxon Simon S   Szittya Gyorgy G   Sorefan Karim K   Moulton Vincent V   Dalmay Tamas T  

The Plant journal : for cell and molecular biology 20110509 2


Plants feature a particularly diverse population of short (s)RNAs, the central component of all RNA silencing pathways. Next generation sequencing techniques enable deeper insights into this complex and highly conserved mechanism and allow identification and quantification of sRNAs. We employed deep sequencing to monitor the sRNAome of developing tomato fruits covering the period between closed flowers and ripened fruits by profiling sRNAs at 10 time-points. It is known that microRNAs (miRNAs) p  ...[more]

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