Transcriptomics

Dataset Information

11

Methylation status in AG- and PG-derived cells and sperm


ABSTRACT: Genomic imprinting describes the expression of a subset of mammalian genes from one parental chromosome. The parent-of-origin specific expression of imprinted genes relies on DNA methylation of CpG-dinucleotides at differentially methylated regions (DMRs) during gametogenesis. We identified the paternally methylated DMRs at mouse chromosome 1 near the imprinted Zdbf2 gene using a methylated-DNA immunoprecipitation-on-chip (meDIP-on-chip) method applied to DNA from parthenogenetic (PG)- and androgenetic (AG)-derived cells and sperm. To identify novel DMRs, genome-wide methylation analysis of three samples were performed using MeDIP and whole genome tiling array.

ORGANISM(S): Mus musculus  

SUBMITTER: Hiroyuki Sasaki   Hidehiko Ogawa  Hitoshi Hiura  Takahiro Arima  Nobuo Yaegashi  Tomohiro Kono  Tokumasa Horiike  Rosalind M John  Yusuke Miyanari  Naoko Miyauchi  Atsushi Sugawara  Yufeng Li 

PROVIDER: E-GEOD-18126 | ArrayExpress | 2010-05-04

SECONDARY ACCESSION(S): GSE18126PRJNA129043

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

A tripartite paternally methylated region within the Gpr1-Zdbf2 imprinted domain on mouse chromosome 1 identified by meDIP-on-chip.

Hiura Hitoshi H   Sugawara Atsushi A   Ogawa Hidehiko H   John Rosalind M RM   Miyauchi Naoko N   Miyanari Yusuke Y   Horiike Tokumasa T   Li Yufeng Y   Yaegashi Nobuo N   Sasaki Hiroyuki H   Kono Tomohiro T   Arima Takahiro T  

Nucleic acids research 20100412 15


The parent-of-origin specific expression of imprinted genes relies on DNA methylation of CpG-dinucleotides at differentially methylated regions (DMRs) during gametogenesis. To date, four paternally methylated DMRs have been identified in screens based on conventional approaches. These DMRs are linked to the imprinted genes H19, Gtl2 (IG-DMR), Rasgrf1 and, most recently, Zdbf2 which encodes zinc finger, DBF-type containing 2. In this study, we applied a novel methylated-DNA immunoprecipitation-on  ...[more]

Similar Datasets

2010-05-05 | E-GEOD-18178 | ArrayExpress
2010-05-04 | E-GEOD-21662 | ArrayExpress
2010-12-21 | E-GEOD-24740 | ArrayExpress
2010-05-15 | E-GEOD-15154 | ArrayExpress
2011-06-06 | E-GEOD-28284 | ArrayExpress
2010-12-21 | E-GEOD-24785 | ArrayExpress
2014-12-15 | E-GEOD-37183 | ArrayExpress
2010-05-15 | E-GEOD-13632 | ArrayExpress
2014-04-01 | E-GEOD-51567 | ArrayExpress
2013-08-12 | E-GEOD-46949 | ArrayExpress