Transcriptomics,Multiomics

Dataset Information

3

IGH analysis in pro-B cells with H3K4me3, H3K4me2 and H3K9ac


ABSTRACT: STAT5 and IL-7 signaling are thought to control B-lymphopoiesis by regulating key transcription factor genes and activating VH gene segments at the Igh locus. Using conditional mutagenesis, we demonstrate that transgenic Bcl2 expression rescued the development of Stat5-deleted pro-B cells by compensating for the loss of Mcl-1. Ebf1 and Pax5 expression as well as VH recombination were normal in Bcl2-rescued pro-B cells lacking STAT5 or IL-7Ra. In agreement with this finding, STAT5-expressing pro-B cells contained little or no active chromatin at most VH genes. In contrast, Igk rearrangements were increased in STAT5- or IL-7Ra-deficient pro-B cells, consistent with direct binding of STAT5 to the intronic iEk enhancer in wild-type pro-B cells. Hence, STAT5 and IL-7 signaling control cell survival and suppress premature Igk recombination in early B-lymphopoiesis. comparison of wt vs Rag2-/- pro-B cells

OTHER RELATED OMICS DATASETS IN: PRJNA117955

ORGANISM(S): Mus musculus  

SUBMITTER: Shane McManus   Meinrad Busslinger 

PROVIDER: E-GEOD-18278 | ArrayExpress | 2010-07-28

SECONDARY ACCESSION(S): GSE18278PRJNA117955

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-18278.README.txt Txt
E-GEOD-18278.idf.txt Idf
E-GEOD-18278.processed.1.zip Processed
E-GEOD-18278.raw.1.zip Raw
E-GEOD-18278.sdrf.txt Txt
Items per page:
1 - 5 of 5
altmetric image

Publications

Role of STAT5 in controlling cell survival and immunoglobulin gene recombination during pro-B cell development.

Malin Stephen S   McManus Shane S   Cobaleda César C   Novatchkova Maria M   Delogu Alessio A   Bouillet Philippe P   Strasser Andreas A   Busslinger Meinrad M  

Nature immunology 20091129 2


STAT5 and interleukin 7 (IL-7) signaling are thought to control B lymphopoiesis by regulating the expression of key transcription factors and by activating variable (V(H)) gene segments at the immunoglobulin heavy-chain (Igh) locus. Using conditional mutagenesis to delete the gene encoding the transcription factor STAT5, we demonstrate that the development of pro-B cells was restored by transgenic expression of the prosurvival protein Bcl-2, which compensated for loss of the antiapoptotic protei  ...[more]

Similar Datasets

2010-07-28 | GSE18278 | GEO
2010-01-01 | S-EPMC2956121 | BioStudies
2011-02-11 | E-GEOD-27074 | ArrayExpress
2011-01-01 | S-EPMC3233979 | BioStudies
2009-01-01 | S-EPMC3057509 | BioStudies
2012-03-29 | E-GEOD-28373 | ArrayExpress
2007-08-23 | E-GEOD-8472 | ArrayExpress
2010-05-20 | E-GEOD-15145 | ArrayExpress
2015-01-01 | S-EPMC4530030 | BioStudies
2015-01-01 | S-EPMC4795263 | BioStudies