Dataset Information


JMJD2B knockdown treatment in normoxia and hypoxia

ABSTRACT: Estrogen receptor alpha plays a critical role in breast cancer and is a major target in endocrine therapy. HIF-1 alpha have been associated with ER alpha and predict a worse outcome. Recent studies indicate that histone demethylase JMJD2B is a HIF-1 alpha target. However, little is known about the biological functions of JMJD2B, especially in breast cancer. To elucidate the mechanism by which JMJD2B reguates gene expression in normoxia and hypoxia, MCF-7 breast cancer cells were depleted forJMJD2B in normoxia and hypoxia. Our results provide insight into JMJD2B regulation of gene expression in breast cancer cells in normoxia and hypoxia. MCF7 cells were subjected to transfection with siRNA controls and two different siRNA oligos against JMJD2B for 24 hours. Cells were treated in normoxia and hypoxia for another 16 hours.

ORGANISM(S): Homo sapiens  

SUBMITTER: Dilair Baban   Francesca M Buffa  Jiannis Ragoussis  Adrian L Harris  Jun Yang  Francesca Buffa 

PROVIDER: E-GEOD-18384 | ArrayExpress | 2010-09-01



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The histone demethylase JMJD2B is regulated by estrogen receptor alpha and hypoxia, and is a key mediator of estrogen induced growth.

Yang Jun J   Jubb Adrian M AM   Pike Luke L   Buffa Francesca M FM   Turley Helen H   Baban Dilair D   Leek Russell R   Gatter Kevin C KC   Ragoussis Jiannis J   Harris Adrian L AL  

Cancer research 20100803 16

Estrogen receptor alpha (ERalpha) plays an important role in breast cancer. Upregulation of HIF-1alpha in ER(alpha)-positive cancers suggests that HIF-1alpha may cooperate with ERalpha to promote breast cancer progression and consequently affect breast cancer treatment. Here, we show the histone demethylase JMJD2B is regulated by both ERalpha and HIF-1alpha, drives breast cancer cell proliferation in normoxia and hypoxia, and epigenetically regulates the expression of cell cycle genes such as CC  ...[more]

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