Transcriptomics

Dataset Information

485

High-throughput sequencing of small RNAs in Vitis vinifera


ABSTRACT: Vitis vinifera endogenous small RNAs Size fractionated small RNA from total RNA extracts of Vitis vinifera leaves, inflorescences, tendrils and small berries were ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

ORGANISM(S): Vitis vinifera  

SUBMITTER: Tamas Dalmay   József Burgyán  Gyorgy Szittya  Laura Miozzi  Vitantonio Pantaleo  Simon Moxon 

PROVIDER: E-GEOD-18405 | ArrayExpress | 2010-04-29

SECONDARY ACCESSION(S): GSE18405SRP002308PRJNA118253

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Identification of grapevine microRNAs and their targets using high-throughput sequencing and degradome analysis.

Pantaleo Vitantonio V   Szittya Gyorgy G   Moxon Simon S   Miozzi Laura L   Moulton Vincent V   Dalmay Tamas T   Burgyan Jozsef J  

The Plant journal : for cell and molecular biology 20100601 6


In plants, microRNAs (miRNAs) comprise one of three classes of small RNAs regulating gene expression at the post-transcriptional level. Many plant miRNAs are conserved, and play a role in development, abiotic stress responses or pathogen responses. However, some miRNAs have only been found in certain species. Here, we use deep-sequencing, computational and molecular methods to identify, profile, and describe conserved and non-conserved miRNAs in four grapevine (Vitis vinifera) tissues. A total o  ...[more]

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