Transcriptomics

Dataset Information

3

Involvement of a chromatin remodeling complex in damage tolerance during DNA replication


ABSTRACT: ATP-dependent chromatin remodeling complexes have been shown to participate in DNA replication in addition to transcription and DNA repair. However, the mechanisms of their involvement in DNA replication remain unclear. Here, we reveal a specific function of the yeast INO80 chromatin remodeling complex in the DNA damage tolerance pathways. Whereas INO80 is necessary for the resumption of replication at forks stalled by methyl methane sulfonate (MMS), it is not required for replication fork collapse after treatment with hydroxyurea (HU). Mechanistically, INO80 regulates DNA damage tolerance during replication through modulation of PCNA (proliferating cell nuclear antigen) ubiquitination and Rad51-mediated processing of recombination intermediates at impeded replication forks. Our findings establish a mechanistic link between INO80 and DNA damage tolerance pathways, indicating that chromatin remodeling is important for accurate DNA replication. INO80 distribution in WT cells was measured.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Yuki M Katou  Katsuhiko Shirahige  Karina B Falbo  Xuetong Shen 

PROVIDER: E-GEOD-18570 | ArrayExpress | 2010-06-20

SECONDARY ACCESSION(S): GSE18570PRJNA120269

REPOSITORIES: GEO, ArrayExpress

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Publications

Involvement of a chromatin remodeling complex in damage tolerance during DNA replication.

Falbo Karina B KB   Alabert Constance C   Katou Yuki Y   Wu Su S   Han Junhong J   Wehr Tammy T   Xiao Jing J   He Xiangwei X   Zhang Zhiguo Z   Shi Yang Y   Shirahige Katsu K   Pasero Philippe P   Shen Xuetong X  

Nature structural & molecular biology 20091025 11


ATP-dependent chromatin remodeling complexes have been shown to participate in DNA replication in addition to transcription and DNA repair. However, the mechanisms of their involvement in DNA replication remain unclear. Here, we reveal a specific function of the yeast INO80 chromatin remodeling complex in the DNA damage tolerance pathways. Whereas INO80 is necessary for the resumption of replication at forks stalled by methyl methane sulfonate (MMS), it is not required for replication fork colla  ...[more]

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