Dataset Information


MiRNA and lung cancer (INCA Project) - 16 RNG25k microarrays

ABSTRACT: To identify putative novel specific targets of mir-210, we overexpressed miR-210 as well as miR-34a and a siRNA targeted against E2F3 in A549 human adenocarcinoma cells by transfecting them with synthetic pre-miRNAs or a synthetic “negative” pre-miRNA as control (miR-Neg). RNA samples were harvested at 48 hours post-transfection and 2 independent experiments were carried out. 48 hours post-transfection, 2 independent experiments performed in dye-swap: miR-210 versus miR-Neg ; miR-34a versus miR-Neg ; si-E2F3 versus miR-Neg ; si-control versus miR-Neg.

ORGANISM(S): Homo sapiens  

SUBMITTER: marie-pierre puissegur   Kevin Lebrigand  Pascal Barbry 

PROVIDER: E-GEOD-18695 | ArrayExpress | 2010-09-30



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miR-210 is overexpressed in late stages of lung cancer and mediates mitochondrial alterations associated with modulation of HIF-1 activity.

Puisségur M-P MP   Mazure N M NM   Bertero T T   Pradelli L L   Grosso S S   Robbe-Sermesant K K   Maurin T T   Lebrigand K K   Cardinaud B B   Hofman V V   Fourre S S   Magnone V V   Ricci J E JE   Pouysségur J J   Gounon P P   Hofman P P   Barbry P P   Mari B B  

Cell death and differentiation 20101001 3

Following the identification of a set of hypoxia-regulated microRNAs (miRNAs), recent studies have highlighted the importance of miR-210 and of its transcriptional regulation by the transcription factor hypoxia-inducible factor-1 (HIF-1). We report here that miR-210 is overexpressed at late stages of non-small cell lung cancer. Expression of miR-210 in lung adenocarcinoma A549 cells caused an alteration of cell viability associated with induction of caspase-3/7 activity. miR-210 induced a loss o  ...[more]

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