Transcriptomics

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Global gene expression analysis during sporulation of the aquatic fungus Blastocladiella emersonii


ABSTRACT: Blastocladiella emersonii life cycle presents a number of drastic biochemical and morphological changes, mainly during two cell differentiation stages: the germination and the sporulation. To investigate the transcriptional changes taking place during the sporulation phase, which culminates with the production of the zoospores, motile cells responsible for the dispersal of the fungus, microarray experiments were performed. Transcription comparisons between cell at the begining of the sporulation phase (zero min.) and at different time points throughout zoospore differentiation were performed in order to associate the gene expression profile with the morphologial changes which culminate with zoospore differentiation. Microarray experiments along the sporulation phase were also realized in the presence of 1% glucose, once such carbohydrate is able to block zoospore differentiation. The results obtained from both sets of experiments were compared in order to understand the glucose effect on sporulation. There are at least 3 biological replicates (independent harvest) and 2 technical replicates of each array (L - left and R - right).

ORGANISM(S): Blastocladiella emersonii  

SUBMITTER: André G Vieira   Suely L Comes  André L.G. Vieira 

PROVIDER: E-GEOD-18718 | ArrayExpress | 2009-10-27

SECONDARY ACCESSION(S): GSE18718PRJNA121519

REPOSITORIES: GEO, ArrayExpress

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Global gene expression analysis during sporulation of the aquatic fungus Blastocladiella emersonii.

Vieira André L G AL   Gomes Suely L SL  

Eukaryotic cell 20091228 3


The Blastocladiella emersonii life cycle presents a number of drastic biochemical and morphological changes, mainly during two cell differentiation stages: germination and sporulation. To investigate the transcriptional changes taking place during the sporulation phase, which culminates with the production of the zoospores, motile cells responsible for the dispersal of the fungus, microarray experiments were performed. Among the 3,773 distinct genes investigated, a total of 1,207 were classified  ...[more]

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