Transcriptomics

Dataset Information

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Mucosal responses of healthy humans to three different probiotic Lactobacillus bacteria


ABSTRACT: Probiotic bacteria, specific representatives of bacterial species that are a common part of the human microbiota, are proposed to deliver health benefits to the consumer by modulation of intestinal function via largely unknown molecular mechanisms. To explore in vivo mucosal responses of healthy adults to probiotics, we obtained transcriptomes in an intervention study following a double-blind placebo-controlled cross-over design. In the mucosa of the proximal small intestine of healthy volunteers, probiotic strains from the species Lactobacillus acidophilus, L. casei and L. rhamnosus each induced differential gene regulatory networks and pathways in the human mucosa. Comprehensive analyses revealed that these transcriptional networks regulate major basal mucosal processes, and uncovered remarkable similarity to response profiles obtained for specific bioactive molecules and drugs. This study elucidates how intestinal mucosa of healthy humans perceive different probiotics and provides avenues for rationally designed tests of clinical applications. Keywords: mucosal response of healthy adult humans to lactic acid bacteria This study was set up according to a randomised double-blind cross-over placebo-controlled design. It contains transcriptional profiles from biopsies from 7 healthy individuals after oral intake of three different Lactobacillus species or placebo control. In total, this study includes data from 7 individuals x 4 treatments=28 arrays.

ORGANISM(S): Homo sapiens  

SUBMITTER: Peter van Baarlen   Fred J Troost  Michiel Kleerebezem  Mark Boekschoten  Cindy van der Meer  Guido Hooiveld  Robert-Jan M Brummer 

PROVIDER: E-GEOD-18741 | ArrayExpress | 2010-09-08

SECONDARY ACCESSION(S): GSE18741PRJNA121653

REPOSITORIES: GEO, ArrayExpress

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Publications

Human mucosal in vivo transcriptome responses to three lactobacilli indicate how probiotics may modulate human cellular pathways.

van Baarlen Peter P   Troost Freddy F   van der Meer Cindy C   Hooiveld Guido G   Boekschoten Mark M   Brummer Robert J M RJ   Kleerebezem Michiel M  

Proceedings of the National Academy of Sciences of the United States of America 20100907


Probiotic bacteria, specific representatives of bacterial species that are a common part of the human microbiota, are proposed to deliver health benefits to the consumer by modulation of intestinal function through largely unknown molecular mechanisms. To explore in vivo mucosal responses of healthy adults to probiotics, we obtained transcriptomes in an intervention study after a double-blind placebo-controlled cross-over design. In the mucosa of the proximal small intestine of healthy volunteer  ...[more]

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