Transcriptomics

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Transcriptome Analysis and Molecular Signature of Human Retinal Pigment Epithelium


ABSTRACT: The retinal pigment epithelium (RPE) is a polarized cell layer that is critical for photoreceptor function and survival. It’s unique relationship to the photoreceptors and its specific physiology makes the RPE a critical determinant of human vision. Therefore we performed global expression profiling of native and cultured human fetal and adult RPE and determined a unique set of highly-expressed genes (called the “signature” set) by comparing the observed RPE gene profiles to the Novartis expression database (SymAtlas: http://wombat.gnf.org/index.html) of 78 tissues. A comparative analysis of transcriptomes from human fetal and adult RPE, primary cultures and commonly-used cell lines was performed. Using selection criteria of at least 10-fold higher expression in each of three RPE preparations, we identified 154 RPE signature genes, which were validated by qRT-PCR analysis in RPE and in an independent set of 11 tissues.

ORGANISM(S): Homo sapiens  

SUBMITTER: Natalia V Strunnikova   Jennifer Barb  Sheldon Miller  Peter Munson 

PROVIDER: E-GEOD-18811 | ArrayExpress | 2010-04-28

SECONDARY ACCESSION(S): GSE18811PRJNA121231

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptome analysis and molecular signature of human retinal pigment epithelium.

Strunnikova N V NV   Maminishkis A A   Barb J J JJ   Wang F F   Zhi C C   Sergeev Y Y   Chen W W   Edwards A O AO   Stambolian D D   Abecasis G G   Swaroop A A   Munson P J PJ   Miller S S SS  

Human molecular genetics 20100401 12


Retinal pigment epithelium (RPE) is a polarized cell layer critical for photoreceptor function and survival. The unique physiology and relationship to the photoreceptors make the RPE a critical determinant of human vision. Therefore, we performed a global expression profiling of native and cultured human fetal and adult RPE and determined a set of highly expressed 'signature' genes by comparing the observed RPE gene profiles to the Novartis expression database (SymAtlas: http://wombat.gnf.org/in  ...[more]

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