Transcriptomics

Dataset Information

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The Sen1 Helicase Prevents Spurious Transcription by Controlling Genome-Wide Transcription Termination by RNAPII


ABSTRACT: RNA Polymerase II (RNAPII) termination for transcripts containing a polyadenylation signal (PAS) is thought to differ mechanistically from termination for PAS-independent RNAPII transcripts such as sn(o)RNAs. In a screen for factors required for PAS-dependent termination, we identified Sen1, a putative helicase known primarily for its role in PAS-independent termination. We show that Sen1 is required for termination on hundreds of protein-coding genes and suppresses cryptic transcription from nucleosome-free regions on a genomic scale. These effects often overlap with but are also often distinct from those caused by Nrd1 depletion, which also impacts termination of protein-coding and cryptic transcripts, including many genic antisense transcripts. Sen1 controls termination through its helicase activity and stimulates recruitment of factors previously implicated in both PAS-dependent (Rna14, Rat1) and PAS-independent (Nrd1) termination. Thus, RNAPII termination for both protein-coding genes and cryptic transcripts is dependent on multiple pathways. The 2 RNAPII datasets were produced in duplicates and the Sen1 and Nrd1 datasets in triplicates (all IP/Input).

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Bernhard Suter   Pierre-Étienne Jacques  Daniel Xia  Timothy R Hughes  Jack F Greenblatt  Carl G de Boer  Corey Nislow  Kyle Tsui  François Robert  Harm van Bakel 

PROVIDER: E-GEOD-19015 | ArrayExpress | 2011-06-01

SECONDARY ACCESSION(S): GSE19015PRJNA121083

REPOSITORIES: GEO, ArrayExpress

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