ABSTRACT: rs09-07_hb6 - hb6 - The aim is to determine downstream target of HB6 transcription factor involved in plant development - Transcriptome on Arabidopsis HB6 over-expressing plants and BPM Knock down plants during development Keywords: wt vs mutant comparison 6 dye-swap - CATMA arrays
Project description:rs10-01_rrm - quadruple rrm experience - What is the role of the RRM protein family in plants? Plants were grown on soil in controlled environment under LD (16 h light/8 h dark) and the rosette leaves (8-leaf stage seedlings) were collected for RNA preparation. Keywords: normal vs transgenic comparison 2 dye-swap - CATMA arrays
Project description:gnp06-03_microtrac Endogenous hairpins-ir71 targets What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in diferent mutants? Lines IR71 KO n°3 and Col-0 were grown on soil until bolting, Flower buds were then harvested and RNA extracted with Qiagen RNeasy Plant mini kit. RNA was stored at -20°C. 3 dye-swap - gene knock out
Project description:What is the physiological function of cytochrome b561 in Arabidopsis thaliana - We want to check which possible changes occur in gene expression by knocking out the AtCytb1 gene. au08-01_cytb561 - cytb1-1 Keywords: gene knock out 4 dye-swap - CATMA arrays
Project description:ra10-01_laccases; laccase mutations. We demonstrated that laccases are involved in lignin polymerisation. Mutants have already been tested on microarrays and there is few differences compared to wild-type. The laccase mutation seems surgical. We possess a new double mutant, called snips, with a semi-dwarf phenotype, and we want to determine its profile. Each mutant was compared to wild type. All plants were harvested at the same developmental stage in the same growth chamber between 10h30 and 11h. 10 dye-swaps. CATMA arrays.
Project description:ra11-04_qtl - study of the transcriptional consequences of a growth qtl candidate (sg1) - We want to compare the transcriptome of Sg1 insertion mutant to a WT background to understand if this candidate has a role in gene expression regulation at the genome-wide level. - We are analysing a QTL named Sg1 quantitatively affecting the shoot growth in Arabidopsis thaliana. Sg1 mutation exhibits a large set of phenotypic consequences (growth, fitness,…), and the penetrance of these defects increase over generations. The CATMA collaboration aims to perform a T-DNA mutant vs WT analysis, with the WT control being derived from the heterozygous T-DNA parent. A first homozygous generation hypotheticaly derived from the heterozygous T-DNA parent and a 4th homozygous generation showing more severe phenotypes are being compared to the WT (dye swap, biological repetitions). Whole plants were harvested 10 DAG at 11am for each genotype. 4 dye-swap - gene knock out
Project description:ra07-05_myb82 - functional characterization of vvmyb82 - Functional characterization of the transcription factor VvMYB82 which is a regulator of the different phenolic compound biosynthesis. - To compare wt with a surexpressor of VvMYB82 (Line A311 = 35s::VvMYB) and wt with a loss of function mutant AtMYB82 2 dye-swap - wt vs mutant comparison
Project description:adt10-01_wip2 - wip overexpressing plants - Identification of genes induced or repressed by WIP genes - The objective is to identify genes responsible for the phenotypes observed in WIP overexpressing lines. WIP transcription factors are involved in reproductive and vegetative development by acting on the remodeling of various tissues. The WIP related processes have a major impact, in particular on reproduction (sex determination, fertilization, development of the seed). The way of action of these transcription factors is probably conserved, their effect depending only on their specificity of expression. Two WIP genes, NTT and TT1, was overexpressed in Arabidopsis thaliana. The objective is to compare the common deregulated genes (and in particular repressed) in the transcriptome of these two overexpressor lines, to highlight their mode of action and the way they act on the tissue in which they are expressed. Keywords: gene knock in (transgenic),normal vs transgenic comparaison 6 dye-swap - CATMA arrays
Project description:gnp_blan06_torpten - overexpression of pten2a - The impact of the TOR pathway on growth and stress responses. - Comparaison control versus overexpressor. The overexpressor has a male phenotype of sterility. 3 dye-swap - gene knock in (transgenic)
Project description:gnp06-02_starch - isd-starch - What is the impact of the absence of different genes related to the starch metabolism on the global gene expression in Arabidopsis leaves. - comparison between several mutant of the starch biosynthesis and the WT, at the end of the day and at the end of the night Keywords: gene knock out 48 dye-swap - CATMA arrays
Project description:au10-07_mpk4gof - mpk4/mpk4 gof vs mpk4/mpk4 vs col0 - Genes regulated by MPK4 pathway. - In order to study of the genes controlled by MPK4 pathway we generate mpk4 complemented lines expressing active MPK4 (MPK4 GOF). For the transcriptome analysis we compare the MPK4 GOF to mpk4/MPK4 (complemented mpk4 mutant with a wt MPK4) and to Col0. Keywords: genotype comparaison 6 dye-swap - CATMA arrays Comparisons of MPK4 GOF to mpk4/MPK4 (complemented mpk4 mutant with a wt MPK4) and to Col0.