Transcriptomics

Dataset Information

6

MLL and Pol2 Chip-chip in interphase and mitotic arrested cells


ABSTRACT: Mixed Lineage Leukemia (MLL) and its metazoan Trithorax orthologs have been linked with the epigenetic maintenance of transcriptional activity. To identify mechanisms by which MLL perpetuates active transcription in dividing cells, we investigated its role during M-phase of the cell cycle. Unlike other chromatin modifying enzymes examined, we found that MLL associates with gene promoters packaged within condensed mitotic chromosomes. Genome-wide location analysis identified a globally rearranged pattern of MLL occupancy during mitosis in a manner favoring genes that were highly transcribed during interphase. Knockdown experiments revealed that MLL retention at gene promoters during mitosis accelerates transcription reactivation following mitotic exit. MLL tethers Menin, RbBP5, and ASH2L to its occupied sites during mitosis, but is dispensable for preserving histone H3K4 methylation. These findings implicate mitotic bookmarking as a component of Trithorax-based gene regulation which may facilitate inheritance of active gene expression states during cell division. anti-MLL ChIP (antibody 456) and anti-pol2 chip (sc-899) in chromatin prepared from interphase and mitotic HeLa cells

ORGANISM(S): Homo sapiens  

SUBMITTER: Christopher Vakoc  C R Vakoc 

PROVIDER: E-GEOD-19155 | ArrayExpress | 2010-05-19

SECONDARY ACCESSION(S): GSE19155PRJNA120709

REPOSITORIES: GEO, ArrayExpress

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Publications

A reconfigured pattern of MLL occupancy within mitotic chromatin promotes rapid transcriptional reactivation following mitotic exit.

Blobel Gerd A GA   Kadauke Stephan S   Wang Eric E   Lau Alan W AW   Zuber Johannes J   Chou Margaret M MM   Vakoc Christopher R CR  

Molecular cell 20091201 6


Mixed lineage leukemia (MLL) and its metazoan Trithorax orthologs have been linked with the epigenetic maintenance of transcriptional activity. To identify mechanisms by which MLL perpetuates active transcription in dividing cells, we investigated its role during M phase of the cell cycle. Unlike other chromatin-modifying enzymes examined, we found that MLL associates with gene promoters packaged within condensed mitotic chromosomes. Genome-wide location analysis identified a globally rearranged  ...[more]

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