Transcriptomics

Dataset Information

5

Transcription profiling by array of Mus musculus expressing Cbfb-MYH11


ABSTRACT: It is known that CBFB-MYH11, the fusion gene generated by inversion of chromosome 16 in human acute myeloid leukemia, is causative for oncogenic transformation. However, the mechanism by which CBFB-MYH11 initiates leukemogenesis is not clear. Previously published reports showed that CBFB-MYH11 dominantly inhibits RUNX1 and CBFB, and such inhibition has been suggested as the mechanism for leukemogenesis. However, knockin mice expressing Cbfb-MYH11 (Cbfb+/MYH11) showed defects in primitive hematopoiesis not seen in Cbfb null (Cbfb-/-) embryos indicating that Cbfb-MYH11 has repression independent activities as well. To identify gene expression changes associated with this novel activity, we compared the gene expression profile in the blood cells of Cbfb+/MYH11 and Cbfb-/- embryonic day 12.5 (E12.5) embryos with that of their wildtype littermates. Cbfb-MYH11 chimeras were mated to C57/Bl6 females to generate Cbfb+/MYH11 (Cbfb+/MYH11) and Cbfb+/+ (WT) embryos. Cbfb+/- x Cbfb+/- matings were used to generate Cbfb+/+ (Cbfb+/+) and Cbfb-/- (Cbfb-/-) embryos. Blood from 8-10 E12.5 embryos of the same genotype was pooled, and RNA was isolated, labeled, and hybridized to Affymetrix Genechip mouse microarray (430 2.0) chips. 4 chips were used for both the Cbfb+/MYH11 and littermate control samples. 3 chips were used for the Cbfb-/- samples and littermate control samples.

REANALYSED by: E-GEOD-19194

ORGANISM(S): Mus musculus  

SUBMITTER: Christopher Pan   Lemlem Alemu  Martha Kirby  P P Liu  Ling Zhao  Stacie Anderson  R K Hyde  Yasuhiko Kamikubo 

PROVIDER: E-GEOD-19194 | ArrayExpress | 2010-04-12

SECONDARY ACCESSION(S): GSE19194PRJNA120677

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Cbfb/Runx1 repression-independent blockage of differentiation and accumulation of Csf2rb-expressing cells by Cbfb-MYH11.

Hyde R Katherine RK   Kamikubo Yasuhiko Y   Anderson Stacie S   Kirby Martha M   Alemu Lemlem L   Zhao Ling L   Liu P Paul PP  

Blood 20091209 7


It is known that CBFB-MYH11, the fusion gene generated by inversion of chromosome 16 in human acute myeloid leukemia, is causative for oncogenic transformation. However, the mechanism by which CBFB-MYH11 initiates leukemogenesis is not clear. Previously published reports showed that CBFB-MYH11 dominantly inhibits RUNX1 and CBFB, and such inhibition has been suggested as the mechanism for leukemogenesis. Here we show that Cbfb-MYH11 caused Cbfb/Runx1 repression-independent defects in both primiti  ...[more]

Similar Datasets

2010-03-31 | GSE19194 | GEO
2015-09-15 | E-GEOD-19194 | ExpressionAtlas
| GSE100061 | GEO
2013-02-19 | E-GEOD-42238 | ArrayExpress
| GSE103367 | GEO
2014-04-05 | E-GEOD-55227 | ArrayExpress
| GSE81992 | GEO
2011-03-29 | E-GEOD-21155 | ArrayExpress
2011-03-29 | GSE21155 | GEO
2013-09-09 | E-GEOD-46044 | ArrayExpress