Transcriptomics

Dataset Information

29

Progranulin A mediated MET signalling is essential for liver morphogenesis in zebrafish


ABSTRACT: Regulation of embryonic liver growth remains largely elusive. Progranulin has been discussed in pathological liver growth; however, the functional role of Pgrn in embryonic liver growth has never been addressed. Knockdown of GrnA, the orthologue of mammalian pgrn in zebrafish, displayed a deficient hepatic outgrowth during hepatogenesis. Expression profiles manifested that pgrn-deficiency impaired hepatogenesis associated with dysregulation of Met signaling. Pgrn regulates hepatic expression of Met was further verified in vitro and in vivo. These results indicate that Pgrn is a novel factor required for embryonic hepatic outgrowth and reveal a novel link between Pgrn and Met signaling. To explore the GrnA induced genomic responses during hepatic outgrowth, mRNA expression profiles were compared from grnA morphants and control embryos using zebrafish 14K oligonucleotide microarray at 72 hpf, when hepatocytes were rapid proliferating.

ORGANISM(S): Danio rerio  

SUBMITTER: Yen-Hsing Li   

PROVIDER: E-GEOD-19211 | ArrayExpress| 2014-05-01

SECONDARY ACCESSION(S): GSE19211PRJNA120825

REPOSITORIES: GEO, ArrayExpress

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Progranulin A-mediated MET signaling is essential for liver morphogenesis in zebrafish.

Li Yen-Hsing YH   Chen Mark Hung-Chih MH   Gong Hong-Yi HY   Hu Shao-Yang SY   Li Ya-Wen YW   Lin Gen-Hwa GH   Lin Ching-Chun CC   Liu Wangta W   Wu Jen-Leih JL  

The Journal of biological chemistry 20101020 52


The mechanism that regulates embryonic liver morphogenesis remains elusive. Progranulin (PGRN) is postulated to play a critical role in regulating pathological liver growth. Nevertheless, the exact regulatory mechanism of PGRN in relation to its functional role in embryonic liver development remains to be elucidated. In our study, the knockdown of progranulin A (GrnA), an orthologue of mammalian PGRN, using antisense morpholinos resulted in impaired liver morphogenesis in zebrafish (Danio rerio)  ...[more]

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