Transcriptomics

Dataset Information

6

Expression profile of the “heat-inducible N-degron” of Nab2 (nab2-td)


ABSTRACT: Gene expression in eukaryotes is an essential process that includes transcription, pre-RNA processing and RNA export. All these steps are coupled and normally, any failure in one step affects the other steps and could cause nuclear mRNA retention. One important player in this interface is the poly(A)-RNA binding protein Nab2, which regulates the poly(A)-tail length of mRNAs protecting their 3’-ends from a second round of polyadenylation and facilitating their nucleo-cytoplasmic export. Interestingly, here we show that Nab2 has additional roles in mRNA transcription elongation, tRNA metabolism and rRNA export. We use Genome-wide analysis of expression of a conditional degron nab2 mutant that allows the depletion of the protein in 15 minutes, to demosntrate that the role of Nab2 in RNAPII transcription and RNAPIII metabolism is not the result of a secondary effect. Our results identify primary targets of nab2 revealing novel functions for Nab2 in transcription and metabolism of most types of RNAs, not only poly(A) mRNAs, indicating that Nab2 function is more ubiquitous than previously anticipated, being a central player in the general and coordinated control of gene expression from transcription elongation to translation initiation. Two and three repeats of the wild type control and the nab2-td mutant respectively at time 0, 30 and 75 minutes after the degron induction.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Cristina González-Aguilera   Andrés Aguilera  Cristina González-Aguilera 

PROVIDER: E-GEOD-19302 | ArrayExpress | 2011-12-01

SECONDARY ACCESSION(S): GSE19302PRJNA120891

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Nab2 functions in the metabolism of RNA driven by polymerases II and III.

González-Aguilera Cristina C   Tous Cristina C   Babiano Reyes R   de la Cruz Jesús J   Luna Rosa R   Aguilera Andrés A  

Molecular biology of the cell 20110616 15


Gene expression in eukaryotes is an essential process that includes transcription, RNA processing, and export. One important player in this interface is the poly(A)(+)-RNA-binding protein Nab2, which regulates the mRNA poly(A)(+)-tail length and export. Here we show that Nab2 has additional roles during mRNA transcription, tRNA metabolism, and ribosomal subunit export. Nab2 is associated with the entire open reading frame of actively transcribed RNA polymerase (RNAP) II and III genes. As a conse  ...[more]

Similar Datasets

2011-12-01 | E-GEOD-26303 | ArrayExpress
2011-12-01 | GSE19302 | GEO
2011-12-01 | GSE26303 | GEO
2015-12-31 | E-GEOD-65225 | ArrayExpress
| PRJNA120891 | ENA
2011-06-28 | E-GEOD-24802 | ArrayExpress
2011-02-23 | E-GEOD-18994 | ArrayExpress
2015-08-13 | E-GEOD-65001 | ArrayExpress
2013-01-31 | E-GEOD-27091 | ArrayExpress
2013-01-11 | E-MTAB-981 | ArrayExpress