Transcriptomics

Dataset Information

182

Screening of genes involved in chromosome segregation in meiosis I


ABSTRACT: Prophase I of male meiosis involves dynamic chromosome segregation processes during early spermatogenesis, including synapsis, meiotic recombination, and cohesion. Genetic defects in genes participating in these processes consistently cause reproduction failure in mice. To identify candidate genes responsible for infertility in humans, we performed expression profiling of mouse spermatogenic cells undergoing meiotic prophase I. Cell fractions enriched in spermatogonia, leptotene/zygotene spermatocytes, or pachytene spermatocytes were separately isolated from mouse testes for RNA extraction. To minimize the contamination of other cell types, we fractionated the testicular cells undergoing the first round of spermatogenesis using Percoll gradient procedure. The cell fractions were characterized by morphological analysis by phase contrast microscopy and Nomarski interference microscopy, and then expression of cell lineage- and spermatogenesis stage-specific genes were examined by RT-PCR. The most enriched fractions for spermatogonia (fraction2 from day8), leptotene/zygotene spermatocytes (fraction5 from day12), and pachytene spermatocytes (fraction8 from day15) were subjected to hybridization on Affymetrix microarrays.

ORGANISM(S): Mus musculus  

SUBMITTER: Hiroki Kurahashi   Hiroshi Kogo  Mariko Taniguchi  Makiko Tsutsumi  Tamae Ohye  Hiroe Kowa  Tatsushi Toda  Hasbaira Bolor  Kouji Yamada  Hidehito Inagaki 

PROVIDER: E-GEOD-19532 | ArrayExpress | 2010-03-31

SECONDARY ACCESSION(S): GSE19532PRJNA122251

REPOSITORIES: GEO, ArrayExpress

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Publications

Screening of genes involved in chromosome segregation during meiosis I: toward the identification of genes responsible for infertility in humans.

Kogo Hiroshi H   Kowa-Sugiyama Hiroe H   Yamada Kouji K   Bolor Hasbaira H   Tsutsumi Makiko M   Ohye Tamae T   Inagaki Hidehito H   Taniguchi Mariko M   Toda Tatsushi T   Kurahashi Hiroki H  

Journal of human genetics 20100326 5


Prophase I of male meiosis during early spermatogenesis involves dynamic chromosome segregation processes, including synapsis, meiotic recombination and cohesion. Genetic defects in the genes that participate in these processes consistently cause reproduction failure in mice. To identify candidate genes responsible for infertility in humans, we performed gene expression profiling of mouse spermatogenic cells undergoing meiotic prophase I. Cell fractions enriched in spermatogonia, leptotene/zygot  ...[more]

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