Transcriptomics

Dataset Information

7

Transcription profiling by array of human estrogen receptor positive breast cancer cell lines after long-term estrogen deprivation


ABSTRACT: Hyperactivation of phosphatidylinositol-3 kinase (PI3K) promotes escape from hormone dependence in estrogen receptor-positive breast cancer. A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. We used gene expression microarrays to identify genes and pathways that are commonly dysregulated in ER+ cell lines with acquired hormone-independent growth. MCF-7, ZR75-1, MDA-361, and HCC-1428 ER+, estrogen-responsive breast cancer cells were cultured under hormone-depleted conditions (10% DCC-FBS) for several months until sustainable hormone-independent cell populations emerged. Parental and long-term estrogen-deprived (LTED) cells were treated with 10% dextran-coated charcoal-treated fetal bovine serum (DCC-FBS) x 24 hrs prior to RNA harvest for array analysis.

REANALYSED by: E-GEOD-19639

ORGANISM(S): Homo sapiens  

SUBMITTER: Todd W Miller   Todd Miller  Carlos Arteaga 

PROVIDER: E-GEOD-19639 | ArrayExpress | 2010-06-23

SECONDARY ACCESSION(S): GSE19639PRJNA122527

REPOSITORIES: GEO, ArrayExpress

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Publications

Hyperactivation of phosphatidylinositol-3 kinase promotes escape from hormone dependence in estrogen receptor-positive human breast cancer.

Miller Todd W TW   Hennessy Bryan T BT   González-Angulo Ana M AM   Fox Emily M EM   Mills Gordon B GB   Chen Heidi H   Higham Catherine C   García-Echeverría Carlos C   Shyr Yu Y   Arteaga Carlos L CL  

The Journal of clinical investigation 20100607 7


Many breast cancers exhibit a degree of dependence on estrogen for tumor growth. Although several therapies have been developed to treat individuals with estrogen-dependent breast cancers, some tumors show de novo or acquired resistance, rendering them particularly elusive to current therapeutic strategies. Understanding the mechanisms by which these cancers develop resistance would enable the development of new and effective therapeutics. In order to determine mechanisms of escape from hormone  ...[more]

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