Transcriptomics

Dataset Information

299

Argonaute Surveillance and Dicer-Independent priRNAs TriggerRNAi and Heterochromatin Formation


ABSTRACT: The assembly of fission yeast pericentromeric heterochromatin and generation of small interfering RNAs (siRNAs) from noncoding centromeric transcripts are mutually dependent processes. How this interdependent positive feedback loop is first triggered is a fundamental unanswered question. Here we show that two distinct Argonaute (Ago1)-dependent pathways mediate small RNA generation. RNA-dependent RNA polymerase complex (RDRC) and Dicer act on specific noncoding RNAs to generate siRNAs by a mechanism that requires the slicer activity of Ago1 but is independent of pre-existing heterochromatin. In the absence of RDRC or Dicer, a distinct class of small RNAs, called primal small RNAs (priRNAs), associate with Ago1. priRNAs are degradation products of abundant transcripts, which bind to Ago1 and target antisense transcripts that result from bidirectional transcription of DNA repeats. Our results suggest that a transcriptome surveillance mechanism based on the random association of RNA degradation products with Argonaute triggers siRNA amplification and heterochromatin assembly within DNA repeats. small RNA profiling in wild type S. pombe cells and in 12 mutant cells

ORGANISM(S): Schizosaccharomyces pombe  

SUBMITTER: Mario Halic   Danesh Moazed 

PROVIDER: E-GEOD-19734 | ArrayExpress | 2010-02-23

SECONDARY ACCESSION(S): GSE19734SRP001978PRJNA122193

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Dicer-independent primal RNAs trigger RNAi and heterochromatin formation.

Halic Mario M   Moazed Danesh D  

Cell 20100201 4


Assembly of fission yeast pericentromeric heterochromatin and generation of small interfering RNAs (siRNAs) from noncoding centromeric transcripts are mutually dependent processes. How this interdependent positive feedback loop is first triggered is a fundamental unanswered question. Here, we show that two distinct Argonaute (Ago1)-dependent pathways mediate small RNA generation. RNA-dependent RNA polymerase complex (RDRC) and Dicer act on specific noncoding RNAs to generate siRNAs by a mechanis  ...[more]

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