Dataset Information


MiRNA profiling by Illumina sequencing technology

ABSTRACT: miRNAs are 19-25 nucleotides long small RNAs now well-known for their regulatory roles in the development and diseases through post-transcriptional and translational controls in a wide range of species. Mammalian hibernation is a physiological process involving dramatic metabolic suppression and cellular reorganization, during which miRNAs may play an important role. We systematically analyzed the miRNAs in the liver of an extreme hibernating species, arctic ground squirrels (Spermophilus parryii), during two stages of hibernation compared to non-hibernating animals by massively parallel Illumina sequencing technology. We identified more than 200 ground squirrel miRNAs including novel miRNAs specific to ground squirrel and a fast-evolving miRNA cluster that also showed significant differential expression during hibernation. Integrating with Agilent miRNA microarray and Real-time PCR results, we identified that mir-211, mir-378, mir-184, mir-200a, and mir-320 were significantly under-expressed during hibernation, whereas mir-144, mir-486, mir-451, mir-142-5p, and mir-1 were over-expressed. Analyses of the their target genes suggested that these miRNAs could play an important role to suppress tumor progression and cell growth during hibernation. Investigation of microRNA changes in arctic ground squirrel livers during Early Arousal(EA), Late Topor(LT), and Post-Reproduction(PR) stages.

ORGANISM(S): Urocitellus parryii  

SUBMITTER: Brian M Barnes   Philipp Khaitovich  Yuting Liu  Wei Chen  Chunxuan Shao  Zheng Yan  Minghua Lu  Jun Yan  Wenchao Hu  Haifang Wang 

PROVIDER: E-GEOD-19807 | ArrayExpress | 2010-11-30



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Genomic analysis of miRNAs in an extreme mammalian hibernator, the Arctic ground squirrel.

Liu Yuting Y   Hu Wenchao W   Wang Haifang H   Lu Minghua M   Shao Chunxuan C   Menzel Corinna C   Yan Zheng Z   Li Ying Y   Zhao Sen S   Khaitovich Philipp P   Liu Mofang M   Chen Wei W   Barnes Brian M BM   Yan Jun J  

Physiological genomics 20100504 1

MicroRNAs (miRNAs) are 19- to 25-nucleotide-long small and noncoding RNAs now well-known for their regulatory roles in gene expression through posttranscriptional and translational controls. Mammalian hibernation is a physiological process involving profound changes in set-points for food consumption, body mass and growth, body temperature, and metabolic rate in which miRNAs may play important regulatory roles. In an initial study, we analyzed miRNAs in the liver of an extreme hibernating specie  ...[more]

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