Transcriptomics

Dataset Information

4

Gene Expression Profiling of siRNA Knock-Down of Six1, Six4 and Myogenin in C2C12


ABSTRACT: Six1, Six4 and Myogenin are transcription factors that are known to be required for skeletal myogenesis. Currently, very little is known about the genes targeted by Six1 and Six4. Gene expression profiling when one or both transcription factors were knock-down by siRNA was performed to identify genes affected by their absence. We also hypothesized that Six1 and Six4 can work in cooperation with the myogenic regulatory factor (MRFs) family of transcription factors, such as Myogenin. Therefore, we performed the same type of experiment where the myogenin was knocked-down by siRNA to identify genes that are possibly regulated by the Six1 or Six4 in conjunction with Myogenin. C2C12 Myoblasts were transfected with siRNA against Six1, Six4, Six1 with Six4, Myogenin, or control 24h before start of differentiation. The cells were allowed to differentiate in differentiation medium for 24h and were harvested for gene expression profiling. Four replicates per siRNA were performed.

ORGANISM(S): Mus musculus  

SUBMITTER: Alphonse Chu  Alexandre Blais 

PROVIDER: E-GEOD-19967 | ArrayExpress | 2010-07-30

SECONDARY ACCESSION(S): GSE19967PRJNA123701

REPOSITORIES: GEO, ArrayExpress

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Publications

Cooperation between myogenic regulatory factors and SIX family transcription factors is important for myoblast differentiation.

Liu Yubing Y   Chu Alphonse A   Chakroun Imane I   Islam Uzma U   Blais Alexandre A  

Nucleic acids research 20100702 20


Precise regulation of gene expression is crucial to myogenesis and is thought to require the cooperation of various transcription factors. On the basis of a bioinformatic analysis of gene regulatory sequences, we hypothesized that myogenic regulatory factors (MRFs), key regulators of skeletal myogenesis, cooperate with members of the SIX family of transcription factors, known to play important roles during embryonic skeletal myogenesis. To this day little is known regarding the exact molecular m  ...[more]

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