Transcriptomics

Dataset Information

7

Diurnal expression data from developing barley caryopses


ABSTRACT: Caryopses of barley (Hordeum vulgare), like all other cereal seeds, are complex sink organs optimized for storage starch accumulation and embryo development. Their development from early stages after pollination to late stages of seed ripening has been studied in great detail. However, information on the caryopses’ diurnal adaptation to changes in light, temperature and alterations in phloem-supplied carbon and nitrogen remained unknown. In this study, we applied the 22K Barley1 GeneChip microarray to investigate diurnal gene regulation events of barley caryopses at 11 to 12 days post anthesis. Developing barley caryopses were harvested at different time-points of a day (at 7:00 AM, 2:00 PM, 5:00 PM and 8:00 PM) including dark samples (6:00 AM and 10:00 PM). Pooled caryopses (form 3 Plants) from the respective time points were used for RNA extraction and hybridization on Affymetrix microarrays (Barley1 GeneChip; GEO accsession GPL1340). Biological replicates were performed.

ORGANISM(S): Hordeum vulgare  

SUBMITTER: Christer G Jansson   Joachim Kilian  Dierk Wanke  Elke Mangelsen 

PROVIDER: E-GEOD-20034 | ArrayExpress | 2010-04-07

SECONDARY ACCESSION(S): GSE20034PRJNA124123

REPOSITORIES: GEO, ArrayExpress

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Publications

Significance of light, sugar, and amino acid supply for diurnal gene regulation in developing barley caryopses.

Mangelsen Elke E   Wanke Dierk D   Kilian Joachim J   Sundberg Eva E   Harter Klaus K   Jansson Christer C  

Plant physiology 20100319 1


The caryopses of barley (Hordeum vulgare), as of all cereals, are complex sink organs optimized for starch accumulation and embryo development. While their early to late development has been studied in great detail, processes underlying the caryopses' diurnal adaptation to changes in light, temperature, and the fluctuations in phloem-supplied carbon and nitrogen have remained unknown. In an attempt to identify diurnally affected processes in developing caryopses at the early maturation phase, we  ...[more]

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