Dataset Information


Expression data from Arabidopsis seed compartments at the bending coyledon stage

ABSTRACT: We collected bending cotyledon seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing linear cotyledon stage embryos. For the purposes of this study we captured 5 compartments: embyro proper, cellularized peripherial endosperm, chalazal endosperm, chalazal seed coat and seed coat, in addition to serial sections encompasing the entire linear cotyledon stage seed. Bending cotyledon stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripherial endosperm, chalazal endosperm, chalazal seed coat, seed coat and whole seeds, 2 biological replicates each).

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Chen Cheng   John J Harada  Meryl M Hashimoto  Jiong Fei  Sandra L Stone  Ryan Christopher Kirkbride  Ryan C Kirkbride  Mark F Belmonte  Robert B Golberg  Julie Pelletier 

PROVIDER: E-GEOD-20039 | ArrayExpress | 2010-02-21



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