Transcriptomics

Dataset Information

9

Genomic mapping of Six1 binding sites during skeletal myoblast differentiation


ABSTRACT: In this study, the C2C12 cell line, a model used to study myogenesis and regeneration, was allowed to differentiate from myoblast precursor cells to myotubes. Cells were harvested at 3 different timepoints to perform ChIP-on-Chip of Six1, which is a key muscle regulator. We identified global loci bound by Six1 during skeletal myoblast differentiation. C2C12 Myoblasts were allowed to differentiate into myotubes. Cells at three timepoints were harvested for ChIP-on-Chip, including myoblasts stage, 24h after differentiation and myotubes (96h after differentiation). Myotubes were detached from the undifferentiated myoblast reserve cells using diluted trypsin. 3 independent biological replicates were used for each time point experiment. A microarray set counts 3 arrays (Custom Arrays A, B and C) for a total of approximately 2.9 million probes.

ORGANISM(S): Mus musculus  

SUBMITTER: Yubing Liu   Alexandre Blais 

PROVIDER: E-GEOD-20150 | ArrayExpress | 2010-07-05

SECONDARY ACCESSION(S): GSE20150PRJNA125575

REPOSITORIES: GEO, ArrayExpress

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Publications

Cooperation between myogenic regulatory factors and SIX family transcription factors is important for myoblast differentiation.

Liu Yubing Y   Chu Alphonse A   Chakroun Imane I   Islam Uzma U   Blais Alexandre A  

Nucleic acids research 20100702 20


Precise regulation of gene expression is crucial to myogenesis and is thought to require the cooperation of various transcription factors. On the basis of a bioinformatic analysis of gene regulatory sequences, we hypothesized that myogenic regulatory factors (MRFs), key regulators of skeletal myogenesis, cooperate with members of the SIX family of transcription factors, known to play important roles during embryonic skeletal myogenesis. To this day little is known regarding the exact molecular m  ...[more]

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