Transcriptomics

Dataset Information

3

ChIP for H3K27me3 in Murine ES Cells: wild type and Ring1B-/- cells


ABSTRACT: Native ChIP on chip for H3K27me3 in murine ES cells comparing WT and Ring1B-/- cells. Paper Abstract: How polycomb group proteins repress gene expression in vivo is not known. Whilst histone modifying activities of the polycomb repressive complexes have been studied extensively, in vitro data has suggested a direct activity of the PRC1 complex in compacting chromatin. Here, we investigate higher-order chromatin compaction of polycomb targets in vivo. We show that polycomb repressive complexes are required to maintain a compact chromatin state at Hox loci in embryonic stem (ES) cells. There is specific decompaction in the absence of PRC2 or PRC1. This is due to PRC1, since decompaction occurs in Ring1B null cells that still have PRC2-mediated H3K27 methylation. Moreover, we show that the ability of Ring1B to restore a compact chromatin state, and to repress Hox gene expression in ES cells, is not dependent on its histone ubiquitination activity. We suggest that Ring1B-mediated chromatin compaction acts to directly limit transcription in vivo. Biological replicates: 3 independently grown, harvested,preplated, micrococcal nuclease digested and ChIP for H3K27me3. 5 Technical replicates.

ORGANISM(S): Mus musculus  

SUBMITTER: Graeme Richard Grimes   Wendy A Bickmore  Ragnhild Eskeland  Graeme R Grimes 

PROVIDER: E-GEOD-20213 | ArrayExpress | 2010-05-14

SECONDARY ACCESSION(S): GSE20213PRJNA125727

REPOSITORIES: GEO, ArrayExpress

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Publications

Ring1B compacts chromatin structure and represses gene expression independent of histone ubiquitination.

Eskeland Ragnhild R   Leeb Martin M   Grimes Graeme R GR   Kress Clémence C   Boyle Shelagh S   Sproul Duncan D   Gilbert Nick N   Fan Yuhong Y   Skoultchi Arthur I AI   Wutz Anton A   Bickmore Wendy A WA  

Molecular cell 20100501 3


How polycomb group proteins repress gene expression in vivo is not known. While histone-modifying activities of the polycomb repressive complexes (PRCs) have been studied extensively, in vitro data have suggested a direct activity of the PRC1 complex in compacting chromatin. Here, we investigate higher-order chromatin compaction of polycomb targets in vivo. We show that PRCs are required to maintain a compact chromatin state at Hox loci in embryonic stem cells (ESCs). There is specific decompact  ...[more]

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