Transcriptomics

Dataset Information

3

STK38 is a Key Regulator of MYC Transcriptional Activity in Human B lymphoma cells


ABSTRACT: Post-translational regulation of the MYC Transcription Factor (TF), including its phosphorylation and ubiquitination, plays an important role in determining cell proliferation and apoptosis and has been implicated in tumorigenesis. Using a computational systems biology approach, followed by biochemical and functional validation, we have characterized the role of the STK38 kinase, an NDR family serine-threonine kinase, as a key modulator of MYC transcriptional activity in human B cells, affecting MYC protein stability in a signal-dependent fashion. Specifically, we show that in human B lymphoma ST486 cells STK38 is a key mediator of BCR pathway signaling, affecting MYC protein turnover and its phosphorylation at Ser62 in kinase-activity-dependent manner. STK38 inactivation abrogates apoptosis following BCR activation while its silencing mediates MYC protein degradation via canonical proteolytic pathways. This suggests that STK38 could provide an effective therapeutic target in MYC-dependent malignancies. ST486 human Burkitt's lymphoma cells were transduced with STK38 shRNA lentiviral vectors.

ORGANISM(S): Homo sapiens  

SUBMITTER: Mariano Javier Alvarez  

PROVIDER: E-GEOD-20229 | ArrayExpress | 2012-01-06

SECONDARY ACCESSION(S): GSE20229PRJNA124165

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2012-01-06 | GSE20229 | GEO
2009-07-27 | E-GEOD-17172 | ArrayExpress
| GSE71724 | GEO
| GSE71721 | GEO
| GSE97133 | GEO
| GSE97132 | GEO
2010-05-27 | E-GEOD-15811 | ArrayExpress
2012-02-01 | E-GEOD-32229 | ArrayExpress
2011-02-04 | E-GEOD-27028 | ArrayExpress
2016-05-09 | PXD003492 | Pride