Genomics

Dataset Information

373

Promoter proximal pausing and its regulation by c-Myc in embryonic stem cells: ChIP-Seq


ABSTRACT: Recruitment of the RNA Polymerase II (Pol II) transcription initiation apparatus to promoters by specific DNA binding transcription factors is well recognized as a key regulatory step in gene expression. We describe here evidence that promoter-proximal pausing is a general feature of transcription by Pol II in embryonic stem (ES) cells, and thus an additional step where regulation of gene expression may occur. We report here that c-Myc, which occupies a third of actively transcribed genes in ES cells and is a key regulator of cellular proliferation, binds P-TEFb and contributes to release of promoter-proximal paused Pol II at these genes. ChIP-seq data for Pol II and additional factors controlling pause release in mouse ES cells.

ORGANISM(S): Mus musculus  

SUBMITTER: Philip A Sharp   Charles Lin  Ryan A Flynn  Peter B Rahl  Scott McCuine  Charles Y Lin  Christopher Burge  Richard A Young  Amy C Seila 

PROVIDER: E-GEOD-20530 | ArrayExpress | 2010-04-02

SECONDARY ACCESSION(S): SRP002008GSE20530PRJNA129865

REPOSITORIES: GEO, ArrayExpress, ENA

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Recruitment of the RNA polymerase II (Pol II) transcription initiation apparatus to promoters by specific DNA-binding transcription factors is well recognized as a key regulatory step in gene expression. We report here that promoter-proximal pausing is a general feature of transcription by Pol II in mammalian cells and thus an additional step where regulation of gene expression occurs. This suggests that some transcription factors recruit the transcription apparatus to promoters, whereas others  ...[more]

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