Transcriptomics

Dataset Information

34

Transcriptome of mouse primary muscle cultures upon dystrophin knockdown by RNAi.


ABSTRACT: Dystrophin was knocked down in primary muscle cultures prepared from C57Bl/10 neonate mice using siRNA targeting dystrophin. Also in parallel, primary muscle cultures treated with siRNA targting luciferase were used as controls. The experiment was designed so that the same cell populations were used for both test and control conditions. This helped avoid the heterogeneity associated with the previous studies where test and control cell populations were non-identical. The experiment resulted in a clear transcriptome of dystrophin deficiency and demonstrated dystrophin as a major organizer of myogensis. Moreover, several interesting experimental targets were identified which can potentially open new lines of investigations. 18 primary muscle cell cultures were prepared on 4 occasions. 7 cultures were treated with siRNAs targeting dystrophin, 7 received siRNA targeting firefly GL2 luciferase (treatment controls) and 4 remained untreated (untreated controls). RNAs were extracted 48 hours after differentiation induction for expression profiling analysis.

ORGANISM(S): Mus musculus  

SUBMITTER: Capucine Trollet  Takis Athanasopoulos   Ian R Graham   George Dickson   Mohammad M Seno   Pingzhao Hu    

PROVIDER: E-GEOD-20548 | ArrayExpress| 2012-03-27

SECONDARY ACCESSION(S): GSE20548PRJNA125305

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptomic analysis of dystrophin RNAi knockdown reveals a central role for dystrophin in muscle differentiation and contractile apparatus organization.

Ghahramani Seno Mohammad M MM   Trollet Capucine C   Athanasopoulos Takis T   Graham Ian R IR   Hu Pingzhao P   Dickson George G  

BMC genomics 20100601


BACKGROUND: Duchenne muscular dystrophy (DMD) is a fatal muscle wasting disorder caused by mutations in the dystrophin gene. DMD has a complex and as yet incompletely defined molecular pathophysiology hindering development of effective ameliorative approaches. Transcriptomic studies so far conducted on dystrophic cells and tissues suffer from non-specific changes and background noise due to heterogeneous comparisons and secondary pathologies. A study design in which a perfectly matched control c  ...[more]

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