Transcriptomics

Dataset Information

4

Stress response


ABSTRACT: Hybridization of one kidney of cortisol treated fish vs. one kidney of control fish. Kidneys were collected from untreated juvenile sea bream (n=4) and from fish, which received for 72h a coconut-oil implant containing 10mg/Kg (fish wet weight) (n=4) cortisol. Experiments were carried out at the University of the Algarve, Portugal in accordance with National legislation for the welfare of animals. Experiments were conducted in two 125 l cylindriconical tanks supplied with a continuous through-flow of oxygenated seawater at 20+1 °C using juvenile sea bream (25 g + 3 g) adapted for 1 week to the experimental conditions. One tank contained 8 untreated fish (control) and the other tank 8 cortisol treated fish and the end of experiments fish were removed form tanks, decapitated and the kidneys rapidly removed and place in RNAlater (Qiagen) at –20 °C. No mortality occurred in the control tank but 2 fish died in the cortisol treated tank.

ORGANISM(S): Sparus aurata  

SUBMITTER: Robert Geisler   Deborah M Power  Elena Sarropoulou  Elena Athina Sarropoulou  Georgios Kotoulas 

PROVIDER: E-GEOD-2064 | ArrayExpress | 2010-06-11

SECONDARY ACCESSION(S): GSE2064PRJNA91313

REPOSITORIES: GEO, ArrayExpress

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Publications

Gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cDNA microarray technology.

Sarropoulou Elena E   Kotoulas Georgios G   Power Deborah M DM   Geisler Robert R  

Physiological genomics 20050726 2


Large-scale gene expression studies were performed for one of the main European aquaculture species, the gilthead sea bream Sparus auratus L. For this purpose, a cDNA microarray containing 10,176 clones from a cDNA library of mixed embryonic and larval stages was constructed. In addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. However, so far, no lar  ...[more]

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