Ontology highlight
ABSTRACT:
SUBMITTER: Eiji Kimura Hideo Matsumura Detlev H Krüger Kentaro Yoshida Ryohei Terauchi Shujun Luo Roberto A Barrero Gary P Schroth Takahiro Fujibe Zayed Albertyn Günter Kahl
PROVIDER: E-GEOD-20682 | ArrayExpress | 2010-09-30
SECONDARY ACCESSION(S): GSE20682PRJNA124943
REPOSITORIES: GEO, ArrayExpress
Matsumura Hideo H Yoshida Kentaro K Luo Shujun S Kimura Eiji E Fujibe Takahiro T Albertyn Zayed Z Barrero Roberto A RA Krüger Detlev H DH Kahl Günter G Schroth Gary P GP Terauchi Ryohei R
PloS one 20100806 8
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High-Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index (barcode) sequences are employed to discriminate tags from different samples. Such barcodes allow researchers to analyze digital tags from transcriptomes of many samples in a single sequencing run by ...[more]