Transcriptomics

Dataset Information

5

The shuttling SR-like proteins Gbp2 and Hrb1 mediate the nuclear export of spliced mRNAs


ABSTRACT: Three shuttling SR-like proteins exist in Saccharomyces cerevisiae, Npl3, Gbp2 and Hrb1, that are involved in the nuclear export of mRNAs. In a screen for genes that regulate the export of Gbp2, we identified novel mutants of the splicing factors PRP8 and PRP17 that lead to severe mislocalization defects for Gbp2 and Hrb1, but not Npl3. Microarray and qRT-PCR analyses show that Gbp2 and Hrb1 preferentially bind to transcripts derived from intron-containing genes. Moreover, in contrast to Npl3, Gbp2 and Hrb1 show genetic and physical interactions with late splicing factors such as Prp17 and Prp43. Further, RNA co-immunoprecipitation experiments reveal that, unlike Npl3, association of Gbp2 and Hrb1 with the mRNA requires splicing, and this in turn is required for their Mex67 recruitment. We propose a model in which Gbp2 and Hrb1 are attached to the mRNAs at late stages of splicing to promote the subsequent export of spliced mRNAs. keyword: RIP-chip RNA-IP of endogenously expressed 3-fold C-terminal myc-tagged Gbp2 and Hrb1 in S288C background cells was each performed once in cells grown to a density of 4x10^7 cells/ml and hybridized against a total (input) RNA reference.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Florian Finkernagel   Birgit Samans  Haijia Wu  Stephanie Heinrich  Alexandra Hackmann  Heike Krebber 

PROVIDER: E-GEOD-20738 | ArrayExpress | 2013-08-30

SECONDARY ACCESSION(S): GSE20738

REPOSITORIES: GEO, ArrayExpress

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