Transcriptomics

Dataset Information

4

Gene expression in tissues of Mus musculus with innate immunity stimulation


ABSTRACT: This is an investigation of whole genome gene expression level in tissues of mice stimulated by LPS, FK565 or LPS + FK565 in vivo and ex vivo. We show that parenteral administration of a pure synthetic Nod1 ligand, FK565, induces site-specific vascular inflammation in mice, which is prominent in aortic root including aortic valves, slight in aorta and absent in other arteries. The degree of respective vascular inflammation is associated with persistent high expression of proinflammatory chemokine/cytokine genes in each tissue in vivo by microarray analysis, and not with Nod1 expression levels. The ex vivo production of proinflammatory chemokine/cytokine by Nod1 ligand is higher in aortic root than in other arteries from normal murine vascular tissues, and also higher in human coronary artery endothelial cells (HCAEC) than in human pulmonary artery endothelial cells (HPAEC), suggesting that site-specific vascular inflammation is at least in part ascribed to an intrinsic nature of the vascular tissue/cell itself. A fourty chip study using total RNA recovered from four isolated tissues of mice which were stimulated by various reagents. Aortic root, pulmonary artery, aorta and spleen of mice in 3 groups: 1) intraperitoneal injection of 20μg of LPS priming only, 2) oral administration of FK565 (100μg) for consecutive days, 3) oral administration of FK565 (100μg) for consecutive days 1 day after LPS priming, at day 2, 4, and 7. And six chip study using total RNA recovered from three isolated vascular tissues of mice which were stimulated by FK565 (10μg/mL) ex vivo.

ORGANISM(S): Mus musculus  

SUBMITTER: Tamami Tanaka   Sagano Onoyama  Kazuyuki Ikeda  Hisanori Nishio  Yukari Fujimoto  Katsuo Sueishi  Shunsuke Kanno  Koichi Fukase  Koichi Kusuhara  Toshiro Hara 

PROVIDER: E-GEOD-20930 | ArrayExpress | 2011-03-01

SECONDARY ACCESSION(S): GSE20930PRJNA124371

REPOSITORIES: GEO, ArrayExpress

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Publications

Nod1 ligands induce site-specific vascular inflammation.

Nishio Hisanori H   Kanno Shunsuke S   Onoyama Sagano S   Ikeda Kazuyuki K   Tanaka Tamami T   Kusuhara Koichi K   Fujimoto Yukari Y   Fukase Koichi K   Sueishi Katsuo K   Hara Toshiro T  

Arteriosclerosis, thrombosis, and vascular biology 20110217 5


OBJECTIVE: The goal of this study was to investigate the effects of stimulants for a nucleotide-binding domain, leucine-rich repeat-containing (NLR) protein family on human artery endothelial cells and murine arteries. METHODS AND RESULTS: Human coronary artery endothelial cells were challenged in vitro with microbial components that stimulate NLRs or Toll-like receptors. We found stimulatory effects of NLR and Toll-like receptor ligands on the adhesion molecule expression and cytokine secretion  ...[more]

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