Transcriptomics

Dataset Information

64

Transcription profiling by array of Arabidopsis mutant for phr1 expressing PHR1 under the control of the rat glucocorticoid receptor after phosphate starvation


ABSTRACT: We performed a transcriptomic analysis of Pi starvation responses in Arabidopsis thaliana (Columbia-0) phr1 mutant plants expressing PHR1 in presence of cicloheximide, that inhibit protein translation, thus preventing any effect of PHR1 on the expression of indirect targets. Results show the primary target genes of PHR1 in the responses to Pi starvation. The analysis was performed in phr1 plants and phr1 plants overexpressing a fusion GR:PHR1 (OXGR:PHR1) whose activity is postranslationally controlled by dexamethasone (DEX), that allows studying gene expression upon PHR1 activation and the concomitant inhibition of translation with cycloheximide (CHX), thus preventing any effect of PHR1 on the expression of indirect targets. To perform this study, phr1 and OXGR:PHR1 phr1 plants were grown for seven days in complete Johnson liquid media (+Pi), 2 days in Pi-lacking media (-Pi) and then supplemented with 5 µM DEX and 10 µM CHX for 6 hours before harvesting. Three independent biological samples of total RNA from shoot and root were hybridized separately.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Regla Bustos   Javier Paz-Ares  Gabriel Castrillo 

PROVIDER: E-GEOD-20955 | ArrayExpress | 2010-12-09

SECONDARY ACCESSION(S): GSE20955PRJNA124443

REPOSITORIES: GEO, ArrayExpress

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Publications

A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis.

Bustos Regla R   Castrillo Gabriel G   Linhares Francisco F   Puga María Isabel MI   Rubio Vicente V   Pérez-Pérez Julian J   Solano Roberto R   Leyva Antonio A   Paz-Ares Javier J  

PLoS genetics 20100909 9


Plants respond to different stresses by inducing or repressing transcription of partially overlapping sets of genes. In Arabidopsis, the PHR1 transcription factor (TF) has an important role in the control of phosphate (Pi) starvation stress responses. Using transcriptomic analysis of Pi starvation in phr1, and phr1 phr1-like (phl1) mutants and in wild type plants, we show that PHR1 in conjunction with PHL1 controls most transcriptional activation and repression responses to phosphate starvation,  ...[more]

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