Transcriptomics

Dataset Information

5

A distinct and unique M2 transcriptional program expressed by tumor-associated macrophages


ABSTRACT: Description This series contains microarrays for tumor associated macrophages (TAMs) purified from 8 week old male C57/BL6N mice, 3 weeks after tumor implantation into the left hind limb. Peritoneal exudates cells (PECs) were also harvested from control C57/BL6N mice. The resulting adherent populations were maintained in culture and resting TAMs and PECs were treated for 4 hours with 100 ng/mL of LPS to promote macrophage activation. RNA isolation and cDNA microarrays: Total RNA was isolated from approximately 20x10e6 PEC and TAM cells using standard Trizol purification protocols (Invitrogen, Carlsbad, CA). Microarrays were manufactured at the NCI, Laboratory of Molecular Technology (Frederick MD). The 10,368 Mm UniGem2 set was printed on poly-lysine coated glass slides and hybridized according to NCI facility protocols. All slides were scanned using as Axon GenePix 4000 scanner (Axon Instruments, Foster city, CA) and resulting data was uploaded for analysis to the mAdb microarray database (http://madb.nci.nih.gov/). Keywords = tumor associated macrophage

ORGANISM(S): Mus musculus  

SUBMITTER: M Sironi   P Saki  B Bottazzi  Lisa M Gangi  A Mantovani  A Sica  L Gangi  T Schioppa  S Biswas  A Saccani 

PROVIDER: E-GEOD-2098 | ArrayExpress | 2010-06-10

SECONDARY ACCESSION(S): GSE2098PRJNA91793

REPOSITORIES: GEO, ArrayExpress

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Publications

A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).

Biswas Subhra K SK   Gangi Lisa L   Paul Saki S   Schioppa Tiziana T   Saccani Alessandra A   Sironi Marina M   Bottazzi Barbara B   Doni Andrea A   Vincenzo Bronte B   Pasqualini Fabio F   Vago Luca L   Nebuloni Manuela M   Mantovani Alberto A   Sica Antonio A  

Blood 20051103 5


To identify the molecular basis underlying the functions of tumor-associated macrophages (TAMs), we characterized the gene expression profile of TAMs isolated from a murine fibrosarcoma in comparison with peritoneal macrophages (PECs) and myeloid suppressor cells (MSCs), using a cDNA microarray technology. Among the differentially expressed genes, 15 genes relevant to inflammation and immunity were validated by real-time polymerase chain reaction (PCR) and protein production. Resting TAMs showed  ...[more]

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