Transcriptomics

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Transcription profiling of human plasma cells from patients with monoclonal gammopathy of undetermined significance, multiple myeloma or plasma-cell leukaemia


ABSTRACT: This series of microarray experiments contains the gene expression profiles of purified plasma cells (PCs) obtained from 7 monoclonal gammopathy of undetermined significance (MGUS), 39 newly diagnosed multiple myeloma (MM) and 6 plasma-cell leukaemia (PCL) patients. PCs were purified from bone marrow Seriess, after red blood cell lysis with 0.86% ammonium chloride, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs was assessed by morphology and flow cytometry and was > 90% in all cases. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A chip following the manufacturer's instructions. After scanning, the images were processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values. Arrays normalization was performed using MAS 5.0 global scaling" procedure, which normalizes the signals of different experiments to the same target intensity (TGT=100).

ORGANISM(S): Homo sapiens  

DISEASE(S): Monoclonal Gammopathy Of Undetermined Significance (mgus),Multiple Myeloma,Plasma Cell Leukemia

SUBMITTER: Antonino Neri  

PROVIDER: E-GEOD-2113 | ArrayExpress | 2007-08-30

SECONDARY ACCESSION(S): GSE2113GDS1067PRJNA91771

REPOSITORIES: GEO, ArrayExpress

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Publications


Multiple myeloma (MM) is the most common form of plasma cell dyscrasia, characterized by a marked heterogeneity of genetic lesions and clinical course. It may develop from a premalignant condition (monoclonal gammopathy of undetermined significance, MGUS) or progress from intramedullary to extramedullary forms (plasma cell leukemia, PCL). To provide insights into the molecular characterization of plasma cell dyscrasias and to investigate the contribution of specific genetic lesions to the biolog  ...[more]

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