Dataset Information


Testing the effects of nitric oxide (oxidative) stress on Caulobacter transcription

ABSTRACT: The goal of this study was to measure the effects of nitric oxide exposure (using DETA NONOate as a nitric oxide donor) on transcription in Caulobacter. Untreated Caulobacter crescentus were grown to a density of 0.3 (at OD660) in PYE medium (pH 7) in rolled culture tubes. DETA-NONOate treated Caulobacter crescentus were grown to a density of 0.3 (at OD660), and then treated with 100 mM DETA NONOate for 30 minutes.

ORGANISM(S): Caulobacter vibrioides  

SUBMITTER: Sean Crosson  

PROVIDER: E-GEOD-21139 | ArrayExpress | 2010-06-20



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Interaction specificity, toxicity and regulation of a paralogous set of ParE/RelE-family toxin-antitoxin systems.

Fiebig Aretha A   Castro Rojas Cyd Marie CM   Siegal-Gaskins Dan D   Crosson Sean S  

Molecular microbiology 20100512 1

Toxin-antitoxin (TA) gene cassettes are widely distributed across bacteria, archaea and bacteriophage. The chromosome of the alpha-proteobacterium, Caulobacter crescentus, encodes eight ParE/RelE-superfamily toxins that are organized into operons with their cognate antitoxins. A systematic genetic analysis of these parDE and relBE TA operons demonstrates that seven encode functional toxins. The one exception highlights an example of a non-functional toxin pseudogene. Chromosomally encoded ParD a  ...[more]

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