Transcriptomics

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Chronic Intermittent Hypoxia Increases Alveolar Surface Area in Adult Mice


ABSTRACT: Obstructive sleep apnea (OSA) leads to increased cardiovascular morbidity and mortality, which have been attributed to intermittent hypoxia (IH). The effects of IH on lung structure and function are unknown. We used a mouse model of chronic IH, which mimics the O2 profile in patients with OSA. We exposed adult C57BL/6J mice to 3 months of IH with an FIO2 nadir of 5%, 60 times/hr during the 12hr light phase. Control mice were exposed to room air. Gene microarrays were performed in a separate subset of animals (IH, n = 5; control, n = 5). Total RNA was isolated from lung tissue using the Trizol Reagent method, gene microarrays were performed with the Affymetrix GeneChip® Mouse Genome 430A 2.0 Array (MG 430A 2.0; Affymetrix, Inc., Santa Clara, CA) and analyzed using Significance Analysis of Microarray (SAM) software.

ORGANISM(S): Mus musculus  

SUBMITTER: Christian Reinke   Dmitry N Grigoryev 

PROVIDER: E-GEOD-21409 | ArrayExpress | 2010-12-31

SECONDARY ACCESSION(S): GSE21409PRJNA126391

REPOSITORIES: GEO, ArrayExpress

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Publications

Chronic intermittent hypoxia induces lung growth in adult mice.

Reinke Christian C   Bevans-Fonti Shannon S   Grigoryev Dmitry N DN   Drager Luciano F LF   Myers Allen C AC   Wise Robert A RA   Schwartz Alan R AR   Mitzner Wayne W   Polotsky Vsevolod Y VY  

American journal of physiology. Lung cellular and molecular physiology 20101203 2


Obstructive sleep apnea (OSA) increases cardiovascular morbidity and mortality, which have been attributed to intermittent hypoxia (IH). The effects of IH on lung structure and function are unknown. We used a mouse model of chronic IH, which mimics the O(2) profile in patients with OSA. We exposed adult C57BL/6J mice to 3 mo of IH with a fraction of inspired oxygen (F(I)(O(2))) nadir of 5% 60 times/h during the 12-h light phase. Control mice were exposed to room air. Lung volumes were measured b  ...[more]

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