Transcriptomics

Dataset Information

41

Melampsora larici-populina gene expression in different tissues of uredinia


ABSTRACT: Laser Capture Microdissection (LCM) was used for expression analysis of three contrasted fungal tissues of uredinia corresponding respectively to spores and sporogenous hyphae, fungal structures in the spongy mesophyll and fungal infection structures in the palisade mesophyll. The array probes were designed from gene models taken from the Joint Genome Institute (JGI, Department of Energy) Melampsora larici-populina genome sequence version 1. The aim of this study was to identify tissue-specific gene expression to gain insights into the genes specifically associated with the biotrophic phase and the sporulation phase of the rust fungus. We performed 9 hybridizations (NimbleGen) with samples derived from Laser Capture Microdissection (LCM) of three contrasted fungal tissues of uredinia, corresponding respectively to spores and sporogenous hyphae, fungal structures in the spongy mesophyll and fungal infection structures in the palisade mesophyll. Three replicates per tissue. All samples were labeled with Cy3.

ORGANISM(S): Melampsora larici-populina  

SUBMITTER: Stéphane Hacquard  Annegret Kohler   Emilie Tisserant   Sébastien Duplessis   Francis Martin   Pascal Frey    

PROVIDER: E-GEOD-21487 | ArrayExpress | 2012-03-27

SECONDARY ACCESSION(S): GSE21487PRJNA126025

REPOSITORIES: GEO, ArrayExpress

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Publications

Laser capture microdissection of uredinia formed by Melampsora larici-populina revealed a transcriptional switch between biotrophy and sporulation.

Hacquard Stéphane S   Delaruelle Christine C   Legué Valérie V   Tisserant Emilie E   Kohler Annegret A   Frey Pascal P   Martin Francis F   Duplessis Sébastien S  

Molecular plant-microbe interactions : MPMI 20101001 10


The foliar rust caused by the basidiomycete Melampsora larici-populina is the main disease affecting poplar plantations in Europe. The biotrophic status of rust fungi is a major limitation to study gene expression of cell or tissue types during host infection. At the uredinial stage, infected poplar leaves contain distinct rust tissues such as haustoria, infection hyphae, and uredinia with sporogenous hyphae and newly formed asexual urediniospores. Laser capture microdissection (LCM) was used to  ...[more]

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