Transcriptomics

Dataset Information

8

Reaeration timecourse from a defined hypoxia model


ABSTRACT: Reaeration timecourse from a defined hypoxia model in Mycobacterium tuberculosis. The mechanism by which mycobacteria return to a replicating state upon re-exposure to favorable conditions is not well understood. In this study, we utilized reaeration from a defined hypoxia model to characterize the adaptive response of Mycobacterium tuberculosis (MTB) following a return to favorable growth conditions. Global transcriptional analysis identified the ~100 gene Reaeration Response, induced relative to both log-phase and hypoxic MTB. This response includes chaperones and proteases, as well as the transcription factor Rv2745c, a Clp protease gene regulator (ClgR) orthologue. During reaeration, genes repressed during hypoxia are also upregulated in a wave of transcription that includes genes crucial to transcription, translation and oxidative phosphorylation. Each reaerating culture compared at multiple timepoints to a sample from the same culture at seven days of hypoxia. Three or more replicates at each timepoint.

ORGANISM(S): Mycobacterium tuberculosis  

SUBMITTER: Tige R Rustad   Gerard A Cangelosi  David R Sherman  Ashley M Sherrid 

PROVIDER: E-GEOD-21590 | ArrayExpress | 2010-07-28

SECONDARY ACCESSION(S): GSE21590PRJNA127035

REPOSITORIES: GEO, ArrayExpress

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Publications

Characterization of a Clp protease gene regulator and the reaeration response in Mycobacterium tuberculosis.

Sherrid Ashley M AM   Rustad Tige R TR   Cangelosi Gerard A GA   Sherman David R DR  

PloS one 20100716 7


Mycobacterium tuberculosis (MTB) enters a non-replicating state when exposed to low oxygen tension, a condition the bacillus encounters in granulomas during infection. Determining how mycobacteria enter and maintain this state is a major focus of research. However, from a public health standpoint the importance of latent TB is its ability to reactivate. The mechanism by which mycobacteria return to a replicating state upon re-exposure to favorable conditions is not understood. In this study, we  ...[more]

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