Transcriptomics

Dataset Information

0

MicroRNA discovery and profiling in human embryonic stem cells by deep sequencing of small RNA libraries


ABSTRACT: We used massively parallel pyrosequencing to discover and characterize microRNAs (miRNAs) expressed in human embryonic stem cells (hESCs). Sequencing of small RNA cDNA libraries derived from undifferentiated hESC and from isogenic differentiating cultures yielded a total of 425,505 high-quality sequence reads. A custom data analysis pipeline delineated expression profiles for 191 previously annotated miRNAs, 36 novel miRNAs highly conserved across vertebrates, and 291 novel candidate miRNAs. A set of nine known and seven novel miRNAs were expressed in undifferentiated hESC and then strongly down-regulated with differentiation. Predicted mRNA targets of these miRNAs exhibited statistically significant enrichment for Gene Ontology functional categories relevant to ESC biology. Our study reveals that hESC express a larger complement of miRNAs than previously appreciated and it provides a resource for future studies of miRNA-mediated regulation in human embryonic stem cells. Two samples were 454 sequenced: undifferentiated human embryonic stem cells and differentiated human embryonic stem cells.

ORGANISM(S): Homo sapiens  

SUBMITTER: Brian R Fritz  Stacia K Wyman   Merav Bar   Evan Kroh   Jerald Radich   Muneesh Tewari   Carol Ware   Angelique Nelson   Robert Gentleman   Rachael Parkin    

PROVIDER: E-GEOD-21722 | ArrayExpress | 2010-05-06

SECONDARY ACCESSION(S): GSE21722PRJNA127361

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2011-03-31 | E-GEOD-27093 | ArrayExpress
2013-01-24 | E-GEOD-43668 | ArrayExpress
2014-02-13 | E-GEOD-42543 | ArrayExpress
2009-02-07 | E-GEOD-14738 | ArrayExpress
2014-02-21 | E-GEOD-55208 | ArrayExpress
2009-07-09 | E-GEOD-16996 | ArrayExpress
2009-09-07 | E-GEOD-15169 | ArrayExpress
2010-07-15 | E-GEOD-22938 | ArrayExpress
2012-12-28 | E-GEOD-34805 | ArrayExpress
2011-03-29 | E-GEOD-24443 | ArrayExpress