Transcriptomics

Dataset Information

4

CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells: Groups 1-3


ABSTRACT: Human Natural Killer (NK) cells comprise two main subsets, CD56bright and CD56dim cells, that differ in function, phenotype and tissue localization. To further dissect the heterogeneity of CD56dim cells, we have performed transcriptome analysis and functional ex vivo characterization of human NK cell subsets according to the expression of markers related to differentiation, migration or competence. Here, we show for the first time that the ability to respond to cytokines or to activating receptors is mutually exclusive in almost all NK cells with the exception of CD56dim CD62L+ cells. Indeed, only these cells combine the ability to produce interferon (IFN)-gamma after cytokines and proliferate in vivo during viral infection with the capacity to kill and produce cytokines upon engagement of activating receptors. Therefore, CD56dim CD62L+ cells represent a unique subset of polyfunctional NK cells. Ex vivo analysis of their function, phenotype, telomere length, frequencies during ageing as well as transfer experiments of NK cell subsets into immunodeficient mice suggest that CD56dim CD62L+ cells represent an intermediate stage of NK cell maturation, which after restimulation can accomplish multiple tasks and further develop into terminally differentiated effectors. Gene expression profiles of FACSAria sorted CD3- CD56bright CD62L+, CD3- CD56dim CD62L+ and CD3- CD56dim CD62L- NK cells from human peripheral blood of three donors were compared using Affymetrix GeneChip Human Genome HG-U133_Plus_2. After total RNA extraction, reverse transcription, cDNA extraction, the biotinylated cRNA was transcribed, fragmented, and 15 µg cRNA hybridized in triplicates for each of the three groups to the GeneChip arrays. Group1: CD3- CD56bright CD62L+,.Group2: CD3- CD56dim CD62L+, Group3: CD3- CD56dim CD62L-. Lists of differentially regulated genes were created using High Performance Chip Data Analysis (HPCDA) with Bioretis database (http://www.bioretis-analysis.de). Worldwide data sharing is possible via Bioretis, please ask the authors.

ORGANISM(S): Homo sapiens  

SUBMITTER: Merlin Luetke-Eversloh   Barbara Morandi  Joachim R. Grün  Andreas Thiel  Kerstin Juelke  Isabela Schmitt-Knosalla  Joachim R Grün  Chiara Romagnani  Monica Killig  Guido Ferlazzo  Eliana Parente 

PROVIDER: E-GEOD-21774 | ArrayExpress | 2010-05-28

SECONDARY ACCESSION(S): GSE21774PRJNA127297

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-21774.README.txt Txt
E-GEOD-21774.eSet.r Other
E-GEOD-21774.idf.txt Idf
E-GEOD-21774.processed.1.zip Processed
E-GEOD-21774.raw.1.zip Raw
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