Dataset Information


Transcription profiling by array of mouse NIH3T3 cells over-expressing PPARg pr EBF1 over time

ABSTRACT: NIH-3T3 cells transduced with either EBF1-, PPARg2- or empty vector were stimulated with hormones to initiate adipocyte differentiation. RNA extraction was done using TriZol at d0, d2, d4 and d10 after stimulation. Samples were handled according to standard affymetrix protocols. Keywords = Adipogenesis, early B-cell factor 1 (EBF1), commitment, differentiation, NIH-3T3, pparg


INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Mus musculus  

SUBMITTER: Robert Mansson  

PROVIDER: E-GEOD-2192 | ArrayExpress | 2007-09-22



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Gene expression analysis suggests that EBF-1 and PPARgamma2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics.

Akerblad Peter P   Månsson Robert R   Lagergren Anna A   Westerlund Simonetta S   Basta Barbro B   Lind Ulrika U   Thelin Anders A   Gisler Ramiro R   Liberg David D   Nelander Sven S   Bamberg Krister K   Sigvardsson Mikael M  

Physiological genomics 20050816 2

Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma2. These experiments revealed that commitment  ...[more]

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